Clone:
REA218
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
Klra5, Ly49e, Ly-49F, Klra6

Specifications for Ly-49E/F Antibody, anti-mouse, REAfinity™

Overview

Clone REA218 recognizes the mouse killer cell lectin-like receptor 5 and 6 (Ly-49E and Ly-49F) antigens, which are type II transmembrane natural killer cell receptors belonging to the Ly-49 family. Members of the Ly-49 family are homodimeric C-type lectin receptors, recognizing MHC class I molecules as ligands and include activating and inhibitory receptor molecules. Ly-49E is expressed abundantly on fetal or immature NK cells and on certain γδ T cells, but at barely detectable frequencies on adult splenic NK cells. Different from other Ly-49 receptors, Ly-49E contains an immunoreceptor tyrosine-based inhibitory motif domain, a charged transmembrane residue and it is present in all mouse strains. Its failure to bind to a variety of soluble or cell bound MHC class I molecules indicates that its natural ligand is not an endogenous class I molecule. IL-2 and IL-15 selectively induce the
de novo
expression of Ly-49E on the majority of mature NK cells. By contrast, IL-4 blocks this IL-2 induced acquisition of Ly-49E. Ly-49F is expressed on subsets of NK cells, NK-T cells, and on a subpopulation of memory T cells in C57BL/6 mice, but not AKR/J, BALB/c, C3H/HeN, DBA/1, or SJL mice. IL-21 selectively down-regulates Ly-49F on mature NK cells.
Additional information: Clone REA218 displays negligible binding to Fc receptors.

Alternative names

Klra5, Ly49e, Ly-49F, Klra6

Detailed product information

Technical specifications

CloneREA218
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenLy-49E/F
Alternative names of antigenKlra5, Ly49e, Ly-49F, Klra6
Molecular mass of antigen [kDa]31
Distribution of antigenNK cells, T cells
Entrez Gene ID16636, 16637
RRIDAB_2652726, AB_2652727, AB_2652728, AB_2652729, AB_2652730, AB_2652731, AB_2652732, AB_2652733, AB_2652734, AB_2652735, AB_2652736, AB_2652737, AB_2652738, AB_2652739, AB_2652740, AB_2652725

Resources for Ly-49E/F Antibody, anti-mouse, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Ly-49E/F Antibody, anti-mouse, REAfinity™

Publications

  1. Gays, F. et al. (2005) Multiple cytokines regulate the NK gene complex-encoded receptor repertoire of mature NK cells and T cells. J. Immunol. 175(5): 2938-2947
  2. Smith, H. R. et al. (1994) Ly-49 multigene family expressed by IL-2-activated NK cells. J. Immunol. 153(3): 1068-1079
  3. Van Beneden, K. et al. (2001) Expression of Ly49E and CD94/NKG2 on fetal and adult NK cells. J. Immunol. 166(7): 4302-4311
  4. Hanke, T. et al. (1999) Direct assessment of MHC class I binding by seven Ly49 inhibitory NK cell receptors. Immunity 11(1): 67-77

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