Clone:
REA673
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC
Alternative names:
LSK

Specifications for Lck pY505 Antibody, anti-human, REAfinity™

Overview

Clone REA673 recognizes the human Lck antigen, phosphorylated at tyrosine 505. Lck is a non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T cells in the thymus and in the function of mature T cells. It is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature α/β TCR. Lck plays a key role in the T cell antigen receptor (TCR)–linked signal transduction pathways and is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated Lck protein to the vicinity of the TCR/CD3 complex. Lck then phosphorylates tyrosines residues within the immunoreceptor tyrosine-based activation motifs of the cytoplasmic tails of the TCR-γ chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Lck also contributes to signaling by other receptor molecules. It associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of Lck, and also plays a role in the IL-2 receptor-linked signaling pathway that controls the T cell proliferative response. Binding of IL-2 to its receptor results in increased activity of Lck. Phosphorylated Tyr-505 associates with the SH2 domain in the amino-terminal half of the protein.
Additional information: Clone REA673 displays negligible binding to Fc receptors.

Alternative names

LSK

Detailed product information

Technical specifications

CloneREA673
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, mouse, other
Cross-reactivitymouse, chicken
AntigenLck pY505
Alternative names of antigenLSK
Molecular mass of antigen [kDa]58
Distribution of antigenT cells, thymocytes
Entrez Gene ID3932
RRIDAB_2652605, AB_2652606, AB_2652607, AB_2652604

Resources for Lck pY505 Antibody, anti-human, REAfinity™

Certificates

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References for Lck pY505 Antibody, anti-human, REAfinity™

Publications

  1. Yasuda, K. et al. (2002) Cutting edge: Fyn is essential for tyrosine phosphorylation of Csk-binding protein/phosphoprotein associated with glycolipid-enriched microdomains in lipid rafts in resting T cells. J. Immunol. 169(6): 2813-2817
  2. Rao, N. et al. (2002) Negative regulation of Lck by Cbl ubiquitin ligase. Proc. Natl. Acad. Sci. U.S.A. 99(6): 3794-3799
  3. Bergman, M. et al. (1992) The human p50csk tyrosine kinase phosphorylates p56lck at Tyr-505 and down regulates its catalytic activity. EMBO J. 11(8): 2919-2924
  4. Salmond, R. J. et al. (2009) T-cell receptor proximal signaling via the Src-family kinases, Lck and Fyn, influences T-cell activation, differentiation, and tolerance. Immunol. Rev. 228(1): 9-22
  5. Perlmutter, R. M. et al. (1988) Structure and expression of lck transcripts in human lymphoid cells. J. Cell. Biochem. 38(2): 117-126

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