Alternative names:
KGF-2

Data and images for Human FGF-10

Figures

Figure 1

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Human FGF-10 activity assay.
The biological activity of Human FGF-10, premium grade was determined by proliferation assay using 4MBr‑5 cells.

Figure 1

Human FGF-10 activity assay.
The biological activity of Human FGF-10, premium grade was determined by proliferation assay using 4MBr‑5 cells.

Figure 2

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SDS-PAGE of Human FGF-10, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 2

SDS-PAGE of Human FGF-10, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 3

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Mass spectrometry analysis (ESI-MS) of Human FGF-10, premium grade.
The peak corresponds to the calculated molecular mass of 19306 Da.

Figure 3

Mass spectrometry analysis (ESI-MS) of Human FGF-10, premium grade.
The peak corresponds to the calculated molecular mass of 19306 Da.

Specifications for Human FGF-10

Overview

Recombinant human FGF-10 (Fibroblast growth factor 10) is used for differentiation of ES/iPS cells. The cytokine is critical during embryogenesis, e.g. FGF-10 is essential for the development of epithelial cells in the lung. Human FGF-10 recombinant protein is developed for use in cell culture, differentiation studies, and functional assays.

Applications

Human FGF-10 can be used for a variety of applications, including:
  • Induction of proliferation and differentiation of epithelial cells.
  • Investigation of FGFR 2 signaling.
  • ES/iPS cell differentiation.
Optimal concentration for a specific application should be determined by a dose-response experiment.

Alternative names

KGF-2

Detailed product information

Background information

Fibroblast growth factor 10 (FGF-10) belongs to the FGF family, which is involved in embryogenesis and adult tissue development and regeneration. The function of FGF-10 partially overlaps with the one of other FGF members such as FGF-7. It is expressed in mesenchymal cells especially in lungs, and signals via FGFR 2. FGF-10 influences the development of several tissues including lungs and adipose tissues, and its activity is inhibited by SHH and BMP-4. Human FGF-10 shares 93% amino acid sequence with mouse FGF-10.

Biological activity

  • Proliferation of 4MBr-5 cells
  • premium grade: ≥ 1×
    10
    4
    U/mg
  • research grade: ≥ 0.5×
    10
    4
    U/mg

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Human FGF-10

Documents and Protocols

Background information

Premium-grade cytokine benefits

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

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