Alternative names:
TRAP, CD154, TNFSF5

Data and images for Human CD40-Ligand

Figures

Figure 1

View details
Human CD40-Ligand activity assay:
The specific activity is determined by proliferation assay using enriched CD19
+
B cells in the presence of the cross-linking antibody and of 50 IU/mL Interleukin 4. Activity of Human CD40-Ligand, premium grade (red line) was compared to another commercially available product (black line).

Figure 1

Human CD40-Ligand activity assay:
The specific activity is determined by proliferation assay using enriched CD19
+
B cells in the presence of the cross-linking antibody and of 50 IU/mL Interleukin 4. Activity of Human CD40-Ligand, premium grade (red line) was compared to another commercially available product (black line).

Figure 2

View details
Human CD40-Ligand activity assay:
The specific activity is determined by
activation assay
using enriched CD19
+
B cells in the presence of the cross-linking antibody and of 50 IU/mL Interleukin 4. Activity of Human CD40-Ligand, premium grade (red line) was compared to another commercially available product (black line).

Figure 2

Human CD40-Ligand activity assay:
The specific activity is determined by
activation assay
using enriched CD19
+
B cells in the presence of the cross-linking antibody and of 50 IU/mL Interleukin 4. Activity of Human CD40-Ligand, premium grade (red line) was compared to another commercially available product (black line).

Figure 3

View details
SDS-PAGE of Human CD40-ligand, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 3

SDS-PAGE of Human CD40-ligand, premium grade
under reduced (R) and non-reduced (NR) conditions.

Figure 4

View details
Human CD40-Ligand biological activity.
Activity of Human CD40-Ligand, premium grade, (red bar) was compared to another commercially available product (black bar).

Figure 4

Human CD40-Ligand biological activity.
Activity of Human CD40-Ligand, premium grade, (red bar) was compared to another commercially available product (black bar).

Figure 5

View details
Mass spectrometry analysis (ESI-MS) of Human CD40-Ligand, premium grade. The major peak corresponds to the calculated molecular mass of 23034 Da. The minor peaks correspond to gluconoylation derivatives as described in Geoghegan, K. F.
et al.
(1999).

Figure 5

Mass spectrometry analysis (ESI-MS) of Human CD40-Ligand, premium grade. The major peak corresponds to the calculated molecular mass of 23034 Da. The minor peaks correspond to gluconoylation derivatives as described in Geoghegan, K. F.
et al.
(1999).

Specifications for Human CD40-Ligand

Overview

Human CD40-Ligand is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human CD40-Ligand can be used for a variety of applications, including:
  • Stimulation of B cell activation and proliferation.
  • Stimulation of dendritic cell maturation.
  • Induction of cytokine production in peripheral blood monocytes and T cells.

Alternative names

TRAP, CD154, TNFSF5

Detailed product information

Background information

CD40-Ligand, also known as CD40L, CD154, TRAP, or TNFSF5 is a member of the TNF superfamily transiently expressed on the surface of activated CD4
+
T lymphocytes. It is either expressed membrane-bound or proteolytically released as a soluble form which comprises 2/3 of the extracellular domain. Its receptor CD40 is found on antigen-presenting cells such as B cells, dendritic cells, and macrophages. CD40L-CD40 interaction is important in T cell-APC (antigen-presenting cell) interaction and is e.g. involved in B cell differentiation and proliferation, isotype class-switching, and protection of B cells from apoptosis.

Biological activity

  • Proliferation of CD19
    +
    B cells
  • premium grade: ≥ 4×
    10
    3
    U/mg

Quality description

Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Resources for Human CD40-Ligand

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Human CD40-Ligand

Publications

  1. Spriggs, M.K. et al. (1992) Recombinant human CD40 ligand stimulates B cell proliferation and immunoglobulin E secretion. J. Exp. Med. 176: 1543-1550

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