Clone:
REA332
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC, ICC
Alternative names:
HLA-DPA1, HLA-DPB1, HLA-DQA1, HLA-DQA2, HLA-DQB1, HLA-DQB2, HLA-DQB3, HLA-DRA, HLA-DRB1

Extended validation for HLA-DR, DP, DQ Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA332
Tü39++
L243-
LN3++
Tü36-
AC122+
REA805-
SK10-
HLADQ1+
Tü169+
REA303+
Cells were incubated with an excess of purified unconjugated HLA-DR, DP, DQ (REA332) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for HLA-DR, DP, DQ. Human peripheral blood mononuclear cells (PBMCs) were stained with HLA-DR, DP, DQ antibodies and plotted against the side scatter. As a control, HLA-DR, DP, DQ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for HLA-DR, DP, DQ. Human peripheral blood mononuclear cells (PBMCs) were stained with HLA-DR, DP, DQ antibodies and plotted against the side scatter. As a control, HLA-DR, DP, DQ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for HLA-DR, DP, DQ. Human peripheral blood mononuclear cells (PBMCs) were stained with HLA-DR, DP, DQ antibodies and plotted against the side scatter. As a control, HLA-DR, DP, DQ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for HLA-DR, DP, DQ. Human peripheral blood mononuclear cells (PBMCs) were stained with HLA-DR, DP, DQ antibodies and plotted against the side scatter. As a control, HLA-DR, DP, DQ antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using HLA-DR, DP, DQ (REA332). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using HLA-DR, DP, DQ (REA332). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using HLA-DR, DP, DQ (REA332). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for HLA-DR, DP, DQ Antibody, anti-human, REAfinity™

Overview

Clone REA332 recognizes all major histocompatibility class (MHC) class II HLA-DR, DP, and most DQ antigens. Expressed on the surface of most nucleated cells, MHC class II molecules play an important role in the immune system. They are essential in the defense against infection and are a main consideration in transplantation medicine. In addition to presenting antigenic peptides from predominantly extracellular sources to CD4
+
T cells, MHC class II molecules also mediate the thymic selection of T helper cells. MHC class II molecules consist of an α and β chain and are transported to endosomal-lysosomal compartments by the invariant chain. In humans, MHC class II molecules are encoded by three different loci, HLA-DR, -DQ, and -DP, which display 70% similarity to each other. Despite the essential function of MHC class II molecules in immune defense against pathogens, some alleles are frequently linked to immune diseases.
Additional information: Clone REA332 displays negligible binding to Fc receptors.

Alternative names

HLA-DPA1, HLA-DPB1, HLA-DQA1, HLA-DQA2, HLA-DQB1, HLA-DQB2, HLA-DQB3, HLA-DRA, HLA-DRB1

Detailed product information

Technical specifications

CloneREA332
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, non-human primate
Cross-reactivity
chimpanzee (
Pan troglodytes
)
,
olive baboon (
Papio anubis
)
,
cynomolgus monkey (
Macaca fascicularis
)
,
common marmoset (
Callithrix jacchus
)
AntigenHLA-DR, DP, DQ
Alternative names of antigenHLA-DPA1, HLA-DPB1, HLA-DQA1, HLA-DQA2, HLA-DQB1, HLA-DQB2, HLA-DQB3, HLA-DRA, HLA-DRB1
Distribution of antigenother
Entrez Gene ID3123, 3122, 3127, 3126, 3125, 16149, 3115, 3113, 3117, 3118, 3119, 3120, 3121
RRIDAB_2752176, AB_2802051, AB_2802045, AB_2819548, AB_2819513, AB_2802068, AB_2802062, AB_2857827, AB_2857820, AB_2652185, AB_2652186, AB_2752195

Resources for HLA-DR, DP, DQ Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for HLA-DR, DP, DQ Antibody, anti-human, REAfinity™

Publications

  1. Denzin, L. et al. (2017) Neutralizing antibody responses to viral infections are linked to the non-classical MHC class II gene H2-Ob. Immunity 47(2): 310-322
  2. van Lith, M. et al. (2010) HLA-DP, HLA-DQ, and HLA-DR have different requirements for invariant chain and HLA-DM. J. Biol. Chem. 285(52): 40800-40808
  3. Franchini, G. et al. (2018)
    HIV vaccine candidate activation of hypoxia and the inflammasome in CD14
    +
    monocytes is associated with a decreased risk of SIVmac251 acquisition.
    Nat Med 24(6): 847-856
  4. Jones, E. Y. et al. (2006) MHC class II proteins and disease: a structural perspective. Nat. Rev. Immunol. 6(4): 271-282
  5. Nishimura, Y. et al. (1998) Peptide-based molecular analyses of HLA class II-associated susceptibility to autoimmune diseases. Int. Rev. Immunol. 17(5–6): 229-262
  6. Hoppstädter, J. et al. (2015) M2 polarization enhances silica nanoparticle uptake by macrophages. Front Pharmacol 6: 55

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