Clone:
22F6
Type of antibody:
Primary antibodies
Isotype:
hamster IgG
Applications:
ICFC, MICS, IF, IHC
Alternative names:
IKZF2, ANF1A2, ZNF1A2, ZNFN1A2

Extended validation for Helios Antibody, anti-human/mouse

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with 22F6
REA829++
736440-
Cells were incubated with an excess of purified unconjugated Helios (22F6) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for Helios Antibody, anti-human/mouse

Overview

The monoclonal antibody 22F6 recognizes mouse and human Helios, which is a zinc-finger transcription factor of the Ikaros family. As well as Ikaros and Aiolos Helios functions as an important regulator of lymphoid development and differentiation and is involved in the control of gene expression. Helios is found on the earliest haematopoietic sites of the embryo, in adult haemopoietic stem cells and on regulatory T cells.

Alternative names

IKZF2, ANF1A2, ZNF1A2, ZNFN1A2

Detailed product information

Technical specifications

Clone22F6
Clonalitymonoclonal
Isotypehamster IgG
Hosthamster
Type of antibodyPrimary antibodies
Specieshuman, mouse, rat
Cross-reactivityrat
AntigenHelios
Alternative names of antigenIKZF2, ANF1A2, ZNF1A2, ZNFN1A2
Molecular mass of antigen [kDa]60
Distribution of antigenT cells, hematopoietic stem and progenitor cells
Entrez Gene ID22807
RRIDAB_2651986, AB_2651987, AB_2651988, AB_2651989, AB_2651990, AB_2651985

Resources for Helios Antibody, anti-human/mouse

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for Helios Antibody, anti-human/mouse

Publications

  1. Gottschalk, R. A. et al. (2012)
    Expression of Helios in peripherally induced Foxp3
    +
    regulatory T cells.
    J. Immunol. 188: 976-980
  2. Kelley, C. M. et al. (1998) Helios, a novel dimerization partner of Ikaros expressed in the earliest hematopoietic progenitors. Curr. Biol. 8(9): 508-515
  3. Thornton, A. M. et al. (2010)
    Expression of Helios, an Ikaros transcription factor family member, differentiates thymic-derived from peripherally induced Foxp3
    +
    T regulatory cells.
    Curr. Biol. 184: 3433-3441
  4. Rebollo, A. et al. (2003) Ikaros, Aiolos and Helios: Transcription regulators and lymphoid malignancies. Immunol. Cell Biol. 81: 171-175

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