gentleMACS™ Dissociator

Name: gentleMACS™ Dissociator
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gentleMACS™ Dissociator

Product overview

The gentleMACS™ Dissociator is a benchtop instrument for the semi-automated dissociation of tissues into single-cell suspensions or thorough homogenates. A single sample or two

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The gentleMACS™ Dissociator

Specifications for gentleMACS™ Dissociator

Overview

The gentleMACS™ Dissociator is a benchtop instrument for the semi-automated dissociation of tissues into single-cell suspensions or thorough homogenates. A single sample or two samples in parallel can be processed. Two types of unique gentleMACS Tubes used with the instrument enable the time-saving and easy dissociation or homogenization of tissues in a closed system. The instrument offers optimized gentleMACS Programs for a variety of specific applications. Special protocols have been developed for various tissues. The standardized tissue dissociation or homogenization procedures make for reliable and reproducible results.

For higher throughput and even more flexibility see also the gentleMACS Octo Dissociator. For fully automated tissue dissociation browse the gentleMACS Octo Dissociator with Heaters.

Detailed product information

Applications

Optimized, pre-set gentleMACS Programs have been developed for easy dissociation of tissues into single-cell suspensions, including:

mouse or human tumor
mouse or rat neonatal heart
neural tissue
mouse spleen, lung, lamina propria, muscle, epidermis, or liver
mouse or human skin
and more

Also, programs have been developed for the homogenization of tissues, for:

total RNA or mRNA isolation from fresh or frozen samples
protein extraction
determination of bacterial or viral load
mitochondria isolation

Resources for gentleMACS™ Dissociator

Documents and Protocols

Brochures/posters

MACS® Sample Preparation - Standardize you tissue dissociation and homogenization

Brochures/posters

gentleMACS™ Dissociators - Start smart – innovative tissue dissociation or homogenization

App notes/customer reports

Modulation of allergic airway inflammation by the histamine H4 receptor: analysis by flow cytometry. Hartwig et al. (2012) MACS&more 14(1).

App notes/customer reports

Identification and enumeration of melanoma-specific tumor-infiltrating lymphocytes and cancer stem cells. Hardt et al. (2012) MACS&more 14(1).

App notes/customer reports

An optimized method for the manufacture of a dendritic cell–based vaccine for translational glioblastoma research. Nava et al. (2013) MACS&more 15(2).

App notes/customer reports

Effector memory T helper cells secrete IFN-γ upon stimulation with cytokines: a role in chronic inflammation. Sattler et al. (2011) MACS&more 13(1).

Protocols

Preparation of single-cell suspensions from mouse spleen without enzymatic treatment

References

gentleMACS Dissociator – selected references

Reviews for gentleMACS™ Dissociator

Dissociate Tissues for Cell Recovery

gentleMACS Dissociator (130-093-235)

This is basically a glorified blender that dissociates any tissue digested with collagenase, to extract infiltrating cells. It can be used for tumors, pancreases, kidneys, heart etc. Some solid tissues are difficult to dissociate by manual agitation, and this is where it comes handy. Plus, who wants to hurt their hands when you have a million tissues to process. We actually performed a yield test and found that tumors dissociated with this instrument gave 5 times higher numbers of infiltrating leukocytes than manually agitated samples.

C Tubes & GentleMACS Dissociator for Tumor Dissociation

gentleMACS Dissociator (130-093-235)

Miltenyi Biotech's C-Tubes are designed to streamline tissue dissociation using the gentleMACS™ Dissociator, and are very helpful in doing so. By using set programs on the dissociator machine, you can select for specific cell types that you wish to isolate. I used these to dissociate tumor tissue in a cell line derivation procedure and received consistent, thoroughly positive results with my final cell lines.

C Tubes & gentleMACS Dissociator for Tumor Dissociation

gentleMACS Dissociator (130-093-235)

Miltenyi Biotech's C-Tubes are designed to streamline tissue dissociation, and are very helpful in doing so. By using set programs on the accompanying dissociator machine, you can select for specific cell types that you wish to isolate. I used these to dissociate tumor tissue in a cell line derivation procedure and received consistent, thoroughly positive results with my final cell lines.

Perfect for Tissue Dissociation

gentleMACS Dissociator (130-093-235)

This is a perfect instrument for tissue dissociation. Once you know it you do not want to dissociate animal tissue without it.

Single Cell Isolation From Various Tissues

gentleMACS Dissociator (130-093-235)

Our lab focused on the clonal heterogeneity in various tissues. As part of our project, need to isolate single cells from various tissues ( liver, lung, brain) from mouse and tumors. The reason we choose MACS dissociator because it has pre-programme optimized for single cell preparations - Lung, brain and tumor.

Perfect For Dissociating Various Tissues

gentleMACS Dissociator (130-093-235)

Our lab digests large amounts of both human and animal tissue for single cell isolation. This machine very quickly dissociates many types of tissue that cannot digest in enzyme buffer alone. We use it for lung, spleen, intestine, and lymph nodes.

gentleMACS Dissociator For Suspending Tough Tissues

gentleMACS Dissociator (130-093-235)

My lab studies CD8 T-cell mediated immunity in the genital mucosa and so we utilize this dissociator to break up evolutionarily tough tissues to free and suspend the cells inside.

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