The Diamond CD133 Isolation Kit was specifically developed for the isolation of highly pure Lin
CD133
+
stem cells from peripheral blood, cord blood, bone marrow, and leukapheresis product by sequential sorting.

Data and images for Diamond CD133 Isolation Kit, human

Figures

Figure 1

CD133
+
Lin
cells were isolated from human PBMCs using the Diamond CD133 Isolation Kit, an LD Column, two MS Columns, a MidiMACS™ Separator, and a MiniMACS™ Separator. Cells were fluorescently stained with CD34-FITC, CD133/2 (293C3)-PE, CD45-APC, and Anti-Biotin-PE and analyzed using the MACSQuant® Analyzer.
PBMCs before separation
Lineage-negative fraction
View details

Figure 1

CD133
+
Lin
cells were isolated from human PBMCs using the Diamond CD133 Isolation Kit, an LD Column, two MS Columns, a MidiMACS™ Separator, and a MiniMACS™ Separator. Cells were fluorescently stained with CD34-FITC, CD133/2 (293C3)-PE, CD45-APC, and Anti-Biotin-PE and analyzed using the MACSQuant® Analyzer.
View details

Figure 1

CD133
+
Lin
cells were isolated from human PBMCs using the Diamond CD133 Isolation Kit, an LD Column, two MS Columns, a MidiMACS™ Separator, and a MiniMACS™ Separator. Cells were fluorescently stained with CD34-FITC, CD133/2 (293C3)-PE, CD45-APC, and Anti-Biotin-PE and analyzed using the MACSQuant® Analyzer.
Isolated CD133
+
Lin
-
cells
View details

Figure 1

CD133
+
Lin
cells were isolated from human PBMCs using the Diamond CD133 Isolation Kit, an LD Column, two MS Columns, a MidiMACS™ Separator, and a MiniMACS™ Separator. Cells were fluorescently stained with CD34-FITC, CD133/2 (293C3)-PE, CD45-APC, and Anti-Biotin-PE and analyzed using the MACSQuant® Analyzer.

Specifications for Diamond CD133 Isolation Kit, human

Overview

The Diamond CD133 Isolation Kit was specifically developed for the isolation of highly pure Lin
CD133
+
stem cells from peripheral blood, cord blood, bone marrow, and leukapheresis product by sequential sorting.

Detailed product information

Detailed separation procedure

The Diamond CD133 MicroBead Kit includes a lineage depletion followed by a CD133 isolation. In the first step, cells are labeled with a cocktail of biotin-conjugated antibodies against lineage-specific antigens. These cells are subsequently labeled with Anti-Biotin MicroBeads. Lineage-negative stem and progenitor cells are obtained by depletion of the magnetically labeled cells. Then CD133
+
cells are isolated from the lineage-negative stem and progenitor cells using CD133 MicroBeads. The resulting population obtained are Lin
-
CD133
+
stem cells.
Diamond CD133 MicroBeads recognize the epitope CD133/1. For control staining of separated cells, the application of CD133/2 (293C3) antibody is recommended.

Downstream applications

Lin
-
CD133
+
stem cells can be used for many applications, including:
  • gene or miRNA expression profiling
  • characterization of undifferentiated stem cells and their developmental potential
  • stimulation of proliferation and maturation by cytokines
  • HSC-CFU assays
  • adoptive transfer and hematopoietic reconstitution studies

Columns

For the first magnetic separation (depletion): LD or autoMACS
®
Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.

References for Diamond CD133 Isolation Kit, human

Publications

  1. Estrada-González, P.K. et al. (2014) Decreased frequency, but normal functional integrity of mesenchymal stromal cells derived from untreated and Imatinib-treated chronic myeloid leukemia patients. Leuk. Res. 5: 594-600

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