CD90.2 MicroBeads were developed for positive selection or depletion of mouse T cells from single-cell suspensions of lymphoid and non-lymphoid tissues or from peripheral blood.

Data and images for CD90.2 MicroBeads, mouse

Figures

Figure 1

A single-cell suspension from mouse spleen was prepared using the gentleMACS™ Dissociator. CD90.2
+
T cells were isolated from this single-cell suspension using the CD90.2 MicroBeads, an autoMACS
®
Column, and autoMACS Pro Separator. Cells were fluorescently stained with REAfinity™ Antibodies CD45-FITC, CD45R(220)-PE-Vio
®
770 and CD90.2-VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Viable leukocytes were gated for analysis based on scatter signals, 7-AAD Staining Solution fluorescence, and CD45 expression.
Before separation
After separation
View details

Figure 1

A single-cell suspension from mouse spleen was prepared using the gentleMACS™ Dissociator. CD90.2
+
T cells were isolated from this single-cell suspension using the CD90.2 MicroBeads, an autoMACS
®
Column, and autoMACS Pro Separator. Cells were fluorescently stained with REAfinity™ Antibodies CD45-FITC, CD45R(220)-PE-Vio
®
770 and CD90.2-VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Viable leukocytes were gated for analysis based on scatter signals, 7-AAD Staining Solution fluorescence, and CD45 expression.
View details

Figure 1

A single-cell suspension from mouse spleen was prepared using the gentleMACS™ Dissociator. CD90.2
+
T cells were isolated from this single-cell suspension using the CD90.2 MicroBeads, an autoMACS
®
Column, and autoMACS Pro Separator. Cells were fluorescently stained with REAfinity™ Antibodies CD45-FITC, CD45R(220)-PE-Vio
®
770 and CD90.2-VioBlue
®
and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Viable leukocytes were gated for analysis based on scatter signals, 7-AAD Staining Solution fluorescence, and CD45 expression.

Specifications for CD90.2 MicroBeads, mouse

Overview

CD90.2 MicroBeads were developed for positive selection or depletion of mouse T cells from single-cell suspensions of lymphoid and non-lymphoid tissues or from peripheral blood.

Detailed product information

Background information

The mouse CD90.2 alloantigen, also known as Thy1.2, is a pan T cell marker for the most common inbred mouse strains. These strains include BALB/c, DBA, CBA/J, C3H, C57BL/6, NZB/-, S3L and others. CD90.2 is expressed on thymocytes, peripheral T cells, on some intraepithelial T cells, and at lower levels on early hematopoietic stem cells in bone marrow.
The mouse CD90.2 antibody does not cross-react with CD90.1 (Thy1.1).

Downstream applications

  • T cells isolated with CD90.2 MicroBeads were used to analyze cytokine expression in a mouse model of lung fibrosis or after adoptive transfer of T cells from wild type and knock-out mice into immunodeficient mice.
  • Furthermore, CD90.2 MicroBeads were used for the isolation of tumor-infiltrating T cells to evaluate their immunotherapeutic potential or functional properties.

Columns

For positive selection: MS, LS, or autoMACS
®
Columns. For depletion: LD or autoMACS Columns.

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