The CD69 MicroBead Kit II was developed for positive selection or depletion of activated T cells or B cells, or NK cells from PBMCs, lymphoid tissues, or cultured cells.

Data and images for CD69 MicroBead Kit II, human

Figures

Figure 1

PBMCs were stimulated for 2 hours with CytoStim. Subsequently, CD69
+
cells were isolated using the CD69 MicroBead Kit II, an LS Column, and a MidiMACS™ Separator. The cells are fluorescently stained with Anti-Biotin-PE, for identification of CD69
+
cells, and CD4-FITC.
Unseparated fraction
Depleted cells
View details

Figure 1

PBMCs were stimulated for 2 hours with CytoStim. Subsequently, CD69
+
cells were isolated using the CD69 MicroBead Kit II, an LS Column, and a MidiMACS™ Separator. The cells are fluorescently stained with Anti-Biotin-PE, for identification of CD69
+
cells, and CD4-FITC.
View details

Figure 1

PBMCs were stimulated for 2 hours with CytoStim. Subsequently, CD69
+
cells were isolated using the CD69 MicroBead Kit II, an LS Column, and a MidiMACS™ Separator. The cells are fluorescently stained with Anti-Biotin-PE, for identification of CD69
+
cells, and CD4-FITC.
Isolated CD69
+
cells
View details

Figure 1

PBMCs were stimulated for 2 hours with CytoStim. Subsequently, CD69
+
cells were isolated using the CD69 MicroBead Kit II, an LS Column, and a MidiMACS™ Separator. The cells are fluorescently stained with Anti-Biotin-PE, for identification of CD69
+
cells, and CD4-FITC.

Specifications for CD69 MicroBead Kit II, human

Overview

The CD69 MicroBead Kit II was developed for positive selection or depletion of activated T cells or B cells, or NK cells from PBMCs, lymphoid tissues, or cultured cells.

Detailed product information

Background information

CD69 is expressed on activated T cells, B cells, and NK cells, but not on resting lymphocytes. CD69 is a cell surface protein which is rapidly up-regulated after lymphocyte activation. CD69 is also expressed on activated macrophages and other cell types including neutrophils, eosinophils, and platelets.
For the isolation of CD69
+
cell subsets, the CD69 MicroBead Kit II can be combined with MACS
®
Cell Isolation Kits or MultiSort Kits.

Detailed separation procedure

CD69
+
cells are labeled with CD69-Biotin and Anti-Biotin MicroBeads.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

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