Clone:
REA386
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
ITGA2B, BDPLT16, BDPLT2, GP2B, GPIIb

Extended validation for CD41a Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA386
HIP8++
CLB-tromb, 6C9+
Cells were incubated with an excess of purified unconjugated CD41a (REA386) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD41a. Human peripheral blood platelets were stained with CD41a antibodies and with a suitable counterstaining. As a control, CD41a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD41a. Human peripheral blood platelets were stained with CD41a antibodies and with a suitable counterstaining. As a control, CD41a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD41a. Human peripheral blood platelets were stained with CD41a antibodies and with a suitable counterstaining. As a control, CD41a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD41a. Human peripheral blood platelets were stained with CD41a antibodies and with a suitable counterstaining. As a control, CD41a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD41a (REA386). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD41a (REA386). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD41a (REA386). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD41a Antibody, anti-human, REAfinity™

Overview

Clone REA386 recognizes the human CD41a antigen, a calcium-dependent complex of CD41/CD61 (GPIIb/IIIa) expressed on normal platelets and megakaryocytes. The CD41/CD61 complex is a receptor for fibronectin, fibrinogen, von Willebrand factor, vitronectin, and thrombospondin and is required for platelet aggregation and clotting. Mutations of either CD41 or CD61 in humans causes Glanzmann’s thrombasthenia. Notably, CD41 is also the first blood-specific marker during
in vitro
differentiation of embryonic stem cells and is expressed on all hematopoietic stem and progenitor cells in the early embryo. However, CD41 expression is downregulated in midgestation embryos, and it is currently thought that CD41 expression on adult hematopoietic stem cells remains switched off throughout adult life.
Additional information: Clone REA386 displays negligible binding to Fc receptors.

Alternative names

ITGA2B, BDPLT16, BDPLT2, GP2B, GPIIb

Detailed product information

Technical specifications

CloneREA386
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, non-human primate
Cross-reactivity
chimpanzee (
Pan troglodytes
)
, baboon, capuchin monkey
AntigenCD41a
Alternative names of antigenITGA2B, BDPLT16, BDPLT2, GP2B, GPIIb
Molecular mass of antigen [kDa]110
Distribution of antigenplatelets, megakaryocytes, stem cells, hematopoietic stem and progenitor cells
Entrez Gene ID3674
RRIDAB_2752178, AB_2784190, AB_2784189, AB_2752223, AB_2752222, AB_2819562, AB_2819532, AB_2857722, AB_2857717, AB_2658042, AB_2658043, AB_2658046, AB_2658047, AB_2658052, AB_2658053, AB_2658054, AB_2658055, AB_2752197

Resources for CD41a Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD41a Antibody, anti-human, REAfinity™

Publications

  1. Gekas, C. et al. (2013) CD41 expression marks myeloid-biased adult hematopoietic stem cells and increases with age. Blood 121(22): 4463-4472
  2. Poncz, M. et al. (1987) Structure of the platelet membrane glycoprotein IIb. Homology to the alpha subunits of the vitronectin and fibronectin membrane receptors. J. Biol. Chem. 262(18): 8476-8482

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