Clone:
REA606
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
CD3e, CD3epsilon, CD3, T3

Extended validation for CD3ε Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA606
145-2C11++
500A2++
145-2C11++
17A2++
REA641++
Cells were incubated with an excess of purified unconjugated CD3ε (REA606) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for CD3ε Antibody, anti-mouse, REAfinity™

Overview

Clone REA606 recognizes the mouse CD3ε antigen, a single-pass type I membrane protein, which is a part of the CD3 complex and a subunit of the TCR complex. The CD3 complex mediates signal transduction in several intracellular pathways and has a role in T cell activation and antigen recognition by binding the peptide/MHC antigen complex. CD3ε is expressed on all mature T lymphocytes, NKT cells, and during the development of thymocytes.
Additional information: Clone REA606 displays negligible binding to Fc receptors.

Alternative names

CD3e, CD3epsilon, CD3, T3

Detailed product information

Technical specifications

CloneREA606
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD3ε
Alternative names of antigenCD3e, CD3epsilon, CD3, T3
Molecular mass of antigen [kDa]19
Distribution of antigenT cells, NKT cells, thymocytes
Entrez Gene ID12501
RRIDAB_2728017, AB_2733114, AB_2751783, AB_2751725, AB_2733115, AB_2752022, AB_2751991, AB_2752029, AB_2657902, AB_2657903, AB_2728040

Resources for CD3ε Antibody, anti-mouse, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD3ε Antibody, anti-mouse, REAfinity™

Publications

  1. Clevers, H. et al. (1988) Characterization and expression of the murine CD3-epsilon gene. Proc. Natl. Acad. Sci. U.S.A. 85(22): 8623-8627
  2. Gold, D. P. et al. (1987) Evolutionary relationship between the T3 chains of the T-cell receptor complex and the immunoglobulin supergene family. Proc. Natl. Acad. Sci. U.S.A. 84(21): 7649-7653
  3. Miescher, G. C. et al. (1989) Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex. Immunol. Lett. 23(2): 113-118
  4. Exley, M. et al. (1991) Structure, assembly and intracellular transport of the T cell receptor for antigen. Semin. Immunol. 3(5): 283-297

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