Clone:
REA429
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
JAM-3, JAM-C, JAM-2

Extended validation for CD323 (JAM3) Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD323 (JAM3) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD323 (JAM3)-PE (REA429, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD323 (JAM3) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD323 (JAM3)-PE (REA429, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD323 (JAM3) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD323 (JAM3)-PE (REA429, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD323 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD323c-PE, clone (REA429). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD323 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD323c-PE, clone (REA429). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD323 (JAM3) Antibody, anti-human, REAfinity™

Overview

Clone REA429 recognizes the human CD323 antigen, a single-pass type I membrane protein, which is also known as junctional adhesion molecule 3 (JAM3) or JAM-C. CD323 is a member of the junctional adhesion molecule family and plays a role in leukocyte migration and cell-cell adhesion. CD323 expression is found on endothelial cells and human platelets. It is expressed at desmosomes but not at tight junctions. CD323 is also expressed at low levels on T cells and is upregulated upon activation.
Additional information: Clone REA429 displays negligible binding to Fc receptors.

Alternative names

JAM-3, JAM-C, JAM-2

Detailed product information

Technical specifications

CloneREA429
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD323 (JAM3)
Alternative names of antigenJAM-3, JAM-C, JAM-2
Molecular mass of antigen [kDa]31
Distribution of antigenendothelial cells, T cells, platelets
Entrez Gene ID83700
RRIDAB_2657469, AB_2657470, AB_2657471, AB_2657472, AB_2657473, AB_2657474, AB_2657475, AB_2657476, AB_2657477, AB_2657478, AB_2657479, AB_2657468

Resources for CD323 (JAM3) Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD323 (JAM3) Antibody, anti-human, REAfinity™

Publications

  1. Arrate, M. P. et al. (2001) Cloning of human junctional adhesion molecule 3 (JAM3) and its identification as the JAM2 counter-receptor. J. Biol. Chem. 276(49): 45826-45832
  2. Cunningham, S. A. et al. (2002) JAM2 interacts with alpha4beta1. Facilitation by JAM3. J. Biol. Chem. 277(31): 27589-27592
  3. Mochida, G. H. et al. (2010) A homozygous mutation in the tight-junction protein JAM3 causes hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts. Am. J. Hum. Genet. 87(6): 882-889

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