Clone:
REA396
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
PECAM1, Pecam

Extended validation for CD31 Antibody, anti-rat, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA369
TLD-3A12++
Cells were incubated with an excess of purified unconjugated CD31 (REA396) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD31. Splenocytes from Wistar rat were stained with CD31 antibodies and with a suitable counterstaining. As a control, CD31 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD31. Splenocytes from Wistar rat were stained with CD31 antibodies and with a suitable counterstaining. As a control, CD31 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD31. Splenocytes from Wistar rat were stained with CD31 antibodies and with a suitable counterstaining. As a control, CD31 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD31. Splenocytes from Wistar rat were stained with CD31 antibodies and with a suitable counterstaining. As a control, CD31 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD31 (REA396). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD31 (REA396). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD31 (REA396). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD31 Antibody, anti-rat, REAfinity™

Overview

Clone REA396 recognizes the rat CD31 antigen, a 130 kDa single-pass type I membrane protein also known as platelet endothelial cell adhesion molecule (PECAM-1). CD31 is a homophilic receptor that is expressed by endothelial cells, platelets, granulocytes, macrophages, dendritic cells, T and B cells, and natural killer cells. There are several spliced variants of CD31 – expressed in a cell-type- and species-specific manner in human, mouse, and rat – that arise as a result of alternative splicing of either the transmembrane, or one of the cytoplasmic tail exons. In addition to its well-known homophilic interaction, a number of putative heterotypic ligands for CD31 have also been identified, including the neutrophil-specific antigen CD177 and the ADP-ribosyl cyclase CD38. The homophilic nature of CD31 facilitates its engagement with other CD31-expressing cells, and this includes leukocyte–leukocyte interactions. For instance, T and B cells need to interact with each other and with antigen-presenting cells to mature or become activated. The presence of CD31 is also crucial in establishing interactions of lymphocytes with the endothelium or with platelets to modulate immune responses at the vascular wall and for the recruitment or extravasation of immune cells in inflamed tissues. CD31 has been shown to inhibit antigen receptor signaling in T and B cells through the action of protein-tyrosine-phosphatases.
Additional information: Clone REA396 displays negligible binding to Fc receptors.

Alternative names

PECAM1, Pecam

Detailed product information

Technical specifications

CloneREA396
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesrat
AntigenCD31
Alternative names of antigenPECAM1, Pecam
Molecular mass of antigen [kDa]74
Distribution of antigenendothelial cells, dendritic cells, granulocytes, B cells, macrophages, platelets, NK cells, T cells
Entrez Gene ID29583
RRIDAB_2727599, AB_2751632, AB_2784128, AB_2727579

Resources for CD31 Antibody, anti-rat, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD31 Antibody, anti-rat, REAfinity™

Publications

  1. Williams, K. C. et al. (1996) PECAM-1 (CD31) expression in the central nervous system and its role in experimental allergic encephalomyelitis in the rat. J. Neurosci. Res. 45(6): 747-757
  2. Sato, I. et al. (2008) Distribution of LYVE-1 and CD31 in postnatal rat masseter muscle. Ann. Anat. 190(4): 329-338
  3. Marszalek, A. et al. (2001) Quantitative immunohistochemical analysis of the expression of CD31 during lung development in the rat. Cells Tissues Organs 169(1): 49-54

Related products for
CD31 Antibody, anti-rat, REAfinity™

2 products available

Seems like you are coming from USA!
Do you want to visit our website in your country?