Clone:
REA447
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
hLAIR1, CD305, LAIR-1

Extended validation for CD305 (LAIR-1) Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD305 (LAIR-1) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD305 (LAIR-1)-PE (REA447, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD305 (LAIR-1) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD305 (LAIR-1)-PE (REA447, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD305 (LAIR-1) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD305 (LAIR-1)-PE (REA447, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD305 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD305-PE, clone (REA447). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD305 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD305-PE, clone (REA447). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD305 (LAIR-1) Antibody, anti-human, REAfinity™

Overview

Clone REA447 recognizes the human CD305 antigen, a single-pass type I membrane protein which is also known as leukocyte-associated immunoglobulin-like receptor 1 (LAIR-1). CD305 contains a single extracellular immunoglobulin-like domain and a cytoplasmic tail containing two immune receptor tyrosine-based inhibitory motifs. It is structurally related to several other inhibitory Ig superfamily members. CD305 is expressed on almost all immune cells, including NK cells, T cells, B cells, monocytes, monocyte-derived dendritic cells, eosinophils, basophils, and mast cells. Besides being expressed on differentiated peripheral blood cells, the receptor is also present on CD34
+
hematopoietic progenitor cells and on the majority of thymocytes. Cross-linking of CD305 on human NK cells delivers a potent inhibitory signal that is capable of inhibiting target cell lysis by resting and activated NK cells. Furthermore, CD305 can inhibit the cytotoxic activity of effector T cells upon CD3 cross-linking or antigen stimulation. In primary B cells, CD305 cross-linking leads to decreased BCR-induced calcium mobilization and down-regulation of Ig and cytokine production.
Additional information: Clone REA447 displays negligible binding to Fc receptors.

Alternative names

hLAIR1, CD305, LAIR-1

Detailed product information

Technical specifications

CloneREA447
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD305 (LAIR-1)
Alternative names of antigenhLAIR1, CD305, LAIR-1
Molecular mass of antigen [kDa]28
Distribution of antigenB cells, dendritic cells, monocytes, NK cells, mast cells, T cells, basophils, eosinophils, thymocytes
Entrez Gene ID3903
RRIDAB_2857836, AB_2657221, AB_2657222, AB_2657225, AB_2657226, AB_2657227, AB_2657228, AB_2657229, AB_2657230, AB_2857839

Resources for CD305 (LAIR-1) Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD305 (LAIR-1) Antibody, anti-human, REAfinity™

Publications

  1. Meyaard, L. et al. (1997) LAIR-1, a novel inhibitory receptor expressed on human mononuclear leukocytes. Immunity 7(2): 283-290
  2. Meyaard, L. (2008) The inhibitory collagen receptor LAIR-1 (CD305). J. Leukoc. Biol. 83(4): 799-803
  3. Perbellini, O. et al. (2014) Clinical significance of LAIR1 (CD305) as assessed by flow cytometry in a prospective series of patients with chronic lymphocytic leukemia. Haematologica 99(5): 881-887

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