Clone:
REA223
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
TCRzeta, CD3D, CD3G, CD3e, CD3z

Extended validation for CD3 Antibody, anti-rat, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA223
G4.18++
1F4++
Cells were incubated with an excess of purified unconjugated CD3 (REA223) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD3 (REA223). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD3 (REA223). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD3 (REA223). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD3 Antibody, anti-rat, REAfinity™

Overview

Clone REA223 recognizes the rat CD3 antigen, a complex of four invariable chains: γ, δ, ε, ζ. CD3 is associated with the T cell receptor (TCR) and is responsible for its signal transduction. It is expressed on mature T cells, thymocytes, a subset of NK cells, and epidermal T cells.
Additional information: Clone REA223 displays negligible binding to Fc receptors.

Alternative names

TCRzeta, CD3D, CD3G, CD3e, CD3z

Detailed product information

Technical specifications

CloneREA223
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesrat
AntigenCD3
Alternative names of antigenTCRzeta, CD3D, CD3G, CD3e, CD3z
Molecular mass of antigen [kDa]75.3(sum of molecular weights of subunits)
Distribution of antigenT cells
Entrez Gene ID25300, 25710, 300678, 315609
RRIDAB_2784112, AB_2657092, AB_2657093, AB_2657094, AB_2657095, AB_2657096, AB_2657097, AB_2657098, AB_2657099, AB_2657102, AB_2657103, AB_2657106, AB_2657107, AB_2751629

Resources for CD3 Antibody, anti-rat, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD3 Antibody, anti-rat, REAfinity™

Publications

  1. Sergent-Tanguy, S. et al. (2006)
    Cell surface antigens on rat neural progenitors and characterization of the CD3
    +
    /CD3
    cell populations.
    Differentiation 74(9-10): 530-541
  2. Caraher, E. M. et al. (2000)
    Flow cytometric analysis of intracellular IFN-gamma, IL-4 and IL-10 in CD3
    +
    4
    +
    T-cells from rat spleen.
    J. Immunol. Methods 244(1-2): 29-40

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