Clone:
REA1197
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
programmed cell death 1 ligand 1, PD-L1, PDCD1L1, PDCD1LG1, PDCD1 ligand 1, B7 homolog 1, B7H1

Extended validation for CD274 (B7-H1) Antibody, anti-human, REAfinity™

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD274 (B7-H1). Monocyte derived dendritic cells (MODCs) were stained with CD274 (B7-H1) antibodies and with a suitable counterstaining. As a control, CD274 (B7-H1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD274 (B7-H1). Monocyte derived dendritic cells (MODCs) were stained with CD274 (B7-H1) antibodies and with a suitable counterstaining. As a control, CD274 (B7-H1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD274 (B7-H1). Monocyte derived dendritic cells (MODCs) were stained with CD274 (B7-H1) antibodies and with a suitable counterstaining. As a control, CD274 (B7-H1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD274 (B7-H1). Monocyte derived dendritic cells (MODCs) were stained with CD274 (B7-H1) antibodies and with a suitable counterstaining. As a control, CD274 (B7-H1) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD274 (B7-H1) (REA1197). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD274 (B7-H1) (REA1197). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD274 (B7-H1) (REA1197). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD274 (B7-H1) Antibody, anti-human, REAfinity™

Overview

Clone REA1197 recognizes the human CD274 antigen, also known as programmed cell death 1 ligand 1 (PD-L1), an immune inhibitory receptor ligand which is involved in the costimulatory signal, essential for T cell proliferation and production of interleukin 10 (IL-10) and interferon γ (IFN-γ).
CD274 is expressed on activated T cells, B cells, dendritic cells (DCs), antigen-presenting cells, keratinocytes, monocytes, and various types of tumor cells. In tissues, CD274 is highly expressed in the heart, skeletal muscle, placenta, and lung and weakly expressed in the thymus, spleen, kidney, and liver.
PD-L1 is a type I transmembrane protein that has immunoglobulin V-like and C-like domains.
Additional information: Clone REA1197 displays negligible binding to Fc receptors.

Alternative names

programmed cell death 1 ligand 1, PD-L1, PDCD1L1, PDCD1LG1, PDCD1 ligand 1, B7 homolog 1, B7H1

Detailed product information

Technical specifications

CloneREA1197
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD274 (B7-H1)
Alternative names of antigenprogrammed cell death 1 ligand 1, PD-L1, PDCD1L1, PDCD1LG1, PDCD1 ligand 1, B7 homolog 1, B7H1
Molecular mass of antigen [kDa]31
Distribution of antigenantigen-presenting cells, B cells, dendritic cells, keratinocytes, monocytes, T cells, tumor cells, thymocytes
Entrez Gene ID29126
RRIDAB_2811392, AB_2811399, AB_2811393, AB_2811400, AB_2811394, AB_2811401, AB_2811395, AB_2811402, AB_2811396, AB_2811403, AB_2811397, AB_2811398

Resources for CD274 (B7-H1) Antibody, anti-human, REAfinity™

Certificates

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References for CD274 (B7-H1) Antibody, anti-human, REAfinity™

Publications

  1. Freeman, G. J. et al. (2000) Engagement of the PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation. J. Exp. Med. 192(7): 1027-1034
  2. Youngnak, P. et al. (2003) Differential binding properties of B7-H1 and B7-DC to programmed death-1. Biochem. Biophys. Res. Commun. 307(3): 672-677
  3. Ahmed, M. et al. (2017) The too many faces of PD-L1: a comprehensive conformational analysis study. Biochemistry 56(40): 5428-5439

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