Clone:
12G5
Type of antibody:
Primary antibodies
Isotype:
mouse IgG2aκ
Applications:
FC
Alternative names:
CXCR4, LAP-3, LCR1, LESTR, NPY3R, WHIM, WHIMS, Fusin

Extended validation for CD184 (CXCR4) Antibody, anti-human

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD184 (CXCR4). Human peripheral blood mononuclear cells (PBMCs) were stained with CD184 (CXCR4) antibodies and with a suitable counterstaining. As a control, CD184 (CXCR4) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD184 (CXCR4). Human peripheral blood mononuclear cells (PBMCs) were stained with CD184 (CXCR4) antibodies and with a suitable counterstaining. As a control, CD184 (CXCR4) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD184 (CXCR4). Human peripheral blood mononuclear cells (PBMCs) were stained with CD184 (CXCR4) antibodies and with a suitable counterstaining. As a control, CD184 (CXCR4) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD184 (CXCR4). Human peripheral blood mononuclear cells (PBMCs) were stained with CD184 (CXCR4) antibodies and with a suitable counterstaining. As a control, CD184 (CXCR4) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD184 (CXCR4). Human peripheral blood mononuclear cells (PBMCs) were stained with CD184 (CXCR4) antibodies and with a suitable counterstaining. As a control, CD184 (CXCR4) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD184 (CXCR4) (12G5). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD184 (CXCR4) (12G5). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD184 (CXCR4) (12G5). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD184 (CXCR4) Antibody, anti-human

Overview

The monoclonal antibody 12G5 recognizes human CD184, also known as CXCR4 or fusin, a 45 kDa seven transmembrane G-protein–linked chemokine receptor for SDF-1. CXCR4 is ubiquitously expressed on blood and tissue cells. It mediates chemotaxis in mature and progenitor blood cells and is important for B lympho- and myelopoiesis and cardiogenesis. It is reported to block CD4-independent HIV-2 infection and CD4-dependent infection by some T cell tropic isolates of HIV-1.

Alternative names

CXCR4, LAP-3, LCR1, LESTR, NPY3R, WHIM, WHIMS, Fusin

Detailed product information

Technical specifications

Clone12G5
Clonalitymonoclonal
Isotypemouse IgG2aκ
Isotype controlIsotype Control Antibody, mouse IgG2a
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
cynomolgus monkey (
Macaca fascicularis
)
,
chimpanzee (
Pan troglodytes
)
,
olive baboon (
Papio anubis
)
AntigenCD184 (CXCR4)
Alternative names of antigenCXCR4, LAP-3, LCR1, LESTR, NPY3R, WHIM, WHIMS, Fusin
Molecular mass of antigen [kDa]40
Distribution of antigenB cells, dendritic cells, endothelial cells, epithelial cells, granulocytes, T cells, megakaryocytes, monocytes, platelets, neurons, stem cells, hematopoietic stem and progenitor cells, mesenchymal stem cells, thymocytes, brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, spleen
Entrez Gene ID7852
RRIDAB_2728021, AB_2802067, AB_2802061, AB_2655780, AB_2655781, AB_2655782, AB_2728044

Resources for CD184 (CXCR4) Antibody, anti-human

Certificates

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References for CD184 (CXCR4) Antibody, anti-human

Publications

  1. Ichiyama, K. et al. (2003) A duodenally absorbable CXC chemokine receptor 4 antagonist, KRH-1636, exhibits a potent and selective anti-HIV-1 activity. Proc. Natl. Acad. Sci. U.S.A. 100: 4185-4190
  2. Berger, E. A. et al. (1999) Chemokine receptors as HIV-1 coreceptors: roles in viral entry, tropism, and disease. Annu. Rev. Immunol. 17: 657-700
  3. Loetscher, P. et al. (2000) Chemokines and their receptors in lymphocyte traffic and HIV infection. Adv. Immunol. 74: 127-180
  4. McKnight, A. et al. (1997) Inhibition of human immunodeficiency virus fusion by a monoclonal antibody to a coreceptor (CXCR4) is both cell type and virus strain dependent. J. Virol. 71(2): 1692-1696

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