Clone:
REA282
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
BSG, 5F7, EMMPRIN, M6, OK, TCSF, OK blood group antigen, Tumor cell-derived collagenase stimulatory factor (TCSF)

Extended validation for CD147 Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD147 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD147-PE (REA282, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD147 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD147-PE (REA282, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD147 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD147-PE (REA282, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD147 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD147-PE, clone (REA282). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD147 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD147-PE, clone (REA282). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD147 Antibody, anti-human, REAfinity™

Overview

Clone REA282 recognizes the human CD147 antigen, a single-pass type I membrane protein which is also known as basigin (BSG) or extracellular matrix metalloproteinase inducer (EMMPRIN). CD147 is expressed by many cell types, including epithelial cells, endothelial cells, leukocytes, and tumor cells. It is present in vascular endothelium in non-neoplastic regions of the brain and in tumor cells, but not in proliferating blood vessels in malignant gliomas. CD147 has been implicated in a variety of physiological and pathological activities. It is best known for its ability to function as extracellular matrix metalloproteinase inducer, but has also been shown to regulate lymphocyte responsiveness, monocarboxylate transporter expression, and spermatogenesis.
Additional information: Clone REA282 displays negligible binding to Fc receptors.

Alternative names

BSG, 5F7, EMMPRIN, M6, OK, TCSF, OK blood group antigen, Tumor cell-derived collagenase stimulatory factor (TCSF)

Detailed product information

Technical specifications

CloneREA282
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD147
Alternative names of antigenBSG, 5F7, EMMPRIN, M6, OK, TCSF, OK blood group antigen, Tumor cell-derived collagenase stimulatory factor (TCSF)
Molecular mass of antigen [kDa]40
Distribution of antigenepithelial cells, endothelial cells
Entrez Gene ID682
RRIDAB_2655195, AB_2655196, AB_2655197, AB_2655198, AB_2655199, AB_2655200, AB_2655201, AB_2819588, AB_2857659, AB_2857634, AB_2857696, AB_2857684, AB_2655192, AB_2655193, AB_2655194, AB_2819603

Resources for CD147 Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD147 Antibody, anti-human, REAfinity™

Publications

  1. Kasinrerk, W. et al. (1992) Human leukocyte activation antigen M6, a member of the Ig superfamily, is the species homologue of rat OX-47, mouse basigin, and chicken HT7 molecule. J. Immunol. 149(3): 847-854
  2. Biswas, C. et al. (1995) The human tumor cell-derived collagenase stimulatory factor (renamed EMMPRIN) is a member of the immunoglobulin superfamily. Cancer Res. 55(2): 434-439
  3. Yurchenko, V. et al. (2006) Dealing with the family: CD147 interactions with cyclophilins. Immunology 117(3): 301-309

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