Anti-mPDCA-1 MicroBeads have been developed for the enrichment of mouse plasmacytoid dendritic cells (PDCs) from lymphoid tissues.

Data and images for Anti-mPDCA-1 MicroBeads, mouse

Figures

Figure 1

Mouse mPDCA-1
+
PDCs were enriched from a mouse spleen cell suspension using Anti-mPDCA-1 MicroBeads, two MS Columns, and a MiniMACS™ Separator. The cells are fluorescently stained with Anti-mPDCA-1-FITC and Anti-Ly-6C-PE. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Spleen cells before separation
Spleen cells depleted of mPDCA-1
+
PDCs
View details

Figure 1

Mouse mPDCA-1
+
PDCs were enriched from a mouse spleen cell suspension using Anti-mPDCA-1 MicroBeads, two MS Columns, and a MiniMACS™ Separator. The cells are fluorescently stained with Anti-mPDCA-1-FITC and Anti-Ly-6C-PE. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Mouse mPDCA-1
+
PDCs were enriched from a mouse spleen cell suspension using Anti-mPDCA-1 MicroBeads, two MS Columns, and a MiniMACS™ Separator. The cells are fluorescently stained with Anti-mPDCA-1-FITC and Anti-Ly-6C-PE. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Enriched mPDCA-1
+
PDCs
View details

Figure 1

Mouse mPDCA-1
+
PDCs were enriched from a mouse spleen cell suspension using Anti-mPDCA-1 MicroBeads, two MS Columns, and a MiniMACS™ Separator. The cells are fluorescently stained with Anti-mPDCA-1-FITC and Anti-Ly-6C-PE. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Anti-mPDCA-1 MicroBeads, mouse

Overview

Anti-mPDCA-1 MicroBeads have been developed for the enrichment of mouse plasmacytoid dendritic cells (PDCs) from lymphoid tissues.

Detailed product information

Background information

The mouse plasmacytoid dendritic cell antigen-1 (mPDCA-1) is specifically expressed on mouse PDCs, a subset of CD11c
+
dendritic cells detected at low frequency in all lymphoid tissues, peripheral blood, and some non-lymphoid tissues. In mouse spleen, bone marrow, and lymph nodes, mPDCA-1 is exclusively expressed on cells which are CD11c
low
, CD45R (B220)
+
,Ly-6C
high
, MHC Class II
low
, CD8a
low/-
, CD80
low/-
, CD86
-
, CD40
-
, CD11b
-
, CD90
-
, CD49b (DX5)
-
, CD3
-
, CD19
-
, i.e. on cells with the phenotype of mouse PDCs. Multi-color fluorescent staining of spleen cells clearly revealed, that all CD11c
+
CD45R (B220)
+
Ly-6C
+
PDCs are mPDCA-1
+
and that mPDCA-1 expression is restricted to PDCs in non-treated mice.

Downstream applications

The isolated PDCs are suitable for studies on their functional properties, e.g. antigen uptake and presentation, crosspriming of cytotoxic T cells, T cell activation or tolerance induction, or T helper cell polarization by PDCs in experimental mouse models of infection and autoimmunity.
Highly pure PDCs were isolated with Anti-mPDCA-1 MicroBeads and have been further analyzed for IDO-dependent tolerance-induction.
1
Anti-mPDCA-1 MicroBeads have also been used to isolate PDCs for subsequent analysis of EBV-induced gene 3 (EBI3) expression and regulation in mouse DC subsets.
2

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

References for Anti-mPDCA-1 MicroBeads, mouse

Publications

  1. Fallarino, F. et al. (2005) Ligand and cytokine dependence of the immunosuppressive pathway of tryptophan catabolism in plasmacytoid dendritic cells. Int. Immunol. 17: 1429-1438
  2. Wirtz, S. et al. (2005) EBV-induced gene 3 transcription is induced by TLR signaling in primary dendritic cells via NF-kappa B activation. J. Immunol. 174: 2814-2824

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