The Anti-Cytokeratin MicroBeads, human have been developed for the positive selection of human carcinoma cells after dissociation of human FFPE tumor tissue.

Data and images for Anti-Cytokeratin MicroBeads, human

Figures

Figure 1

Human carcinoma cells were purified from a mixture of dissociated FFPE tumor cells spiked in dissociated FFPE spleen cells using Anti-Cytokeratin MicroBeads, human. Isolation was performed on an autoMACS
®
Pro Separator. The cells were fluorescently stained with Anti-Cytokeratin-FITC, Anti-Vimentin-APC, and CD235a (Glycophorin A)-PE, and DAPI and analyzed by flow cytometry using the MACSQuant
®
Analyzer.
Aggregates were excluded from the analysis based on scatter signals and single cells were gated on DAPI fluorescence.
Before separation
After separation
View details

Figure 1

Human carcinoma cells were purified from a mixture of dissociated FFPE tumor cells spiked in dissociated FFPE spleen cells using Anti-Cytokeratin MicroBeads, human. Isolation was performed on an autoMACS
®
Pro Separator. The cells were fluorescently stained with Anti-Cytokeratin-FITC, Anti-Vimentin-APC, and CD235a (Glycophorin A)-PE, and DAPI and analyzed by flow cytometry using the MACSQuant
®
Analyzer.
Aggregates were excluded from the analysis based on scatter signals and single cells were gated on DAPI fluorescence.
View details

Figure 1

Human carcinoma cells were purified from a mixture of dissociated FFPE tumor cells spiked in dissociated FFPE spleen cells using Anti-Cytokeratin MicroBeads, human. Isolation was performed on an autoMACS
®
Pro Separator. The cells were fluorescently stained with Anti-Cytokeratin-FITC, Anti-Vimentin-APC, and CD235a (Glycophorin A)-PE, and DAPI and analyzed by flow cytometry using the MACSQuant
®
Analyzer.
Aggregates were excluded from the analysis based on scatter signals and single cells were gated on DAPI fluorescence.

Specifications for Anti-Cytokeratin MicroBeads, human

Overview

The Anti-Cytokeratin MicroBeads, human have been developed for the positive selection of human carcinoma cells after dissociation of human FFPE tumor tissue.

Detailed product information

Background information

Worldwide, FFPE tissues are considered the standard specimen for histopathological procedures and retrospective studies aiming to investigate human solid cancers.
Tumor cells in biopsy samples are usually surrounded by numerous noncancer cells, including tumor-infiltrating leukocytes (TILs) and fibroblasts. The presence of these cells potentially hamper the analysis of neoplastic cells.
An effective enrichment of the tumor ratio is therefore of utmost importance.
Most malignant cells, which originate in epithelial tissue express cytokeratins 7/8/18/19 and can be recognized by an anti-cytokeratin antibody
1
. The Anti-Cytokeratin MicroBeads, human guarantee an effective isolation of human carcinoma cells after dissociation of human FFPE tumor tissue.

Downstream applications

Purified human carcinoma cells isolated via MACS
®
Technology can be used for sensitive downstream analyses, such as DNA sequencing by Next Generation Sequencing, flow cytometry, and immunofluorescence (IF).

Columns

MS or autoMACS
®
Columns.

References for Anti-Cytokeratin MicroBeads, human

Publications

  1. Heatley, M. et al. (1995) Cytokeratin intermediate filament expression in benign and malignant breast disease. J. Clin. Pathol. 48: 26-32

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