CD133 MicroBead Kit – Hematopoietic Tissue, human

Name: CD133 MicroBead Kit – Hematopoietic Tissue, human
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CD133 MicroBead Kit – Hematopoietic Tissue, human

The CD133 MicroBead Kit - Hematopoietic Tissue has been developed for easy one-step isolation of CD133

⁺

hematopoietic or endothelial stem and progenitor cells from hematopoietic tissues such as PBMCs, bone marrow or cord blood.

Product data and images for CD133 MicroBead Kit – Hematopoietic Tissue, human

Figures

Figure 1

CD133

⁺

hematopoietic progenitor cells were isolated from non-mobilized human PBMCs using the CD133 MicroBead Kit - Hematopoietic Tissue, labeled with CD34-FITC (# 130-081-001) and CD133/2 (293C3)-PE (# 130-090-853) and analyzed by flow cytometry. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Unseparated fraction

After separation

Figure 1

CD133

⁺

hematopoietic progenitor cells were isolated from non-mobilized human PBMCs using the CD133 MicroBead Kit - Hematopoietic Tissue, labeled with CD34-FITC (# 130-081-001) and CD133/2 (293C3)-PE (# 130-090-853) and analyzed by flow cytometry. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 1

CD133

⁺

hematopoietic progenitor cells were isolated from non-mobilized human PBMCs using the CD133 MicroBead Kit - Hematopoietic Tissue, labeled with CD34-FITC (# 130-081-001) and CD133/2 (293C3)-PE (# 130-090-853) and analyzed by flow cytometry. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 2

Comparison of CD133

⁺

cell isolations from a debris-rich sample using (A) the CD133 MicroBead Kit (# 130-050-801) or (B) the CD133 MicroBead Kit – Hematopoietic Tissue (# 130-100-830), demonstrating reduced debris carry-over with the latter.

A:	B:
View details CD133 MicroBead Kit – Hematopoietic Tissue, human Figure 2 Comparison of CD133 ⁺ cell isolations from a debris-rich sample using (A) the CD133 MicroBead Kit (# 130-050-801) or (B) the CD133 MicroBead Kit – Hematopoietic Tissue (# 130-100-830), demonstrating reduced debris carry-over with the latter.	View details CD133 MicroBead Kit – Hematopoietic Tissue, human Figure 2 Comparison of CD133 ⁺ cell isolations from a debris-rich sample using (A) the CD133 MicroBead Kit (# 130-050-801) or (B) the CD133 MicroBead Kit – Hematopoietic Tissue (# 130-100-830), demonstrating reduced debris carry-over with the latter.

Specifications for CD133 MicroBead Kit – Hematopoietic Tissue, human

Overview

The CD133 MicroBead Kit - Hematopoietic Tissue has been developed for easy one-step isolation of CD133

⁺

hematopoietic or endothelial stem and progenitor cells from hematopoietic tissues such as PBMCs, bone marrow or cord blood.

Detailed product information

Background information

The CD133 MicroBead Kit – Hematopoietic Tissue is a magnetic labeling system designed for the positive selection of CD133

⁺

cells. It allows the single-step isolation of endothelial and hematopoietic stem and progenitor cells.

The CD133 molecule is a 5-transmembrane cell surface antigen with a molecular weight of 117 kDa. In the hematopoietic system, CD133 expression is restricted to a subset of CD34bright stem and progenitor cells in human fetal liver, bone marrow, cord blood, and peripheral blood. Isolated from hematopoietic sources, CD133

⁺

cells can become adherent and are reported to become CD133

^–

during culture. The CD34

⁺

CD133

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cell population, which includes CD34

⁺

CD38

^–

cells, was shown to be capable of repopulating NOD/ SCID mice. In addition, the CD133 antigen is also expressed on circulating endothelial progenitor cells. The CD133 MicroBead Kit – Hematopoietic Tissue has been optimized for the isolation of CD133

⁺

cells of hematopoietic or endothelial origin. For the isolation of CD133

⁺

cells from solid tissues, e.g., the purification of cancer stem cells from solid tumors, we recommend the CD133 MicroBead Kit – Tumor Tissue (# 130-100-857).

Columns

For positive selection: MS, LS, XS, or autoMACS

^®

Columns. For depletion: LD, D, or autoMACS Columns.

Resources for CD133 MicroBead Kit – Hematopoietic Tissue, human

Documents and Protocols

Brochures/posters

Solutions for cutting-edge human hematopoietic stem cell research

Data and images