Discover this optimized workflow for isolating and characterizing human and mouse dendritic cells (DCs). It provides the ability to isolate and analyze CD11c+ DCs and their subsets. Plus, our PepTivator® Technology provides the peptide pools for antigen loading.
DCs are the sentinels of the immune system and are especially located at the interface between the body and external environment. Isolation of DCs from different tissues can be performed by using the combination of gentleMACS™ Octo Dissociator with Heaters, the appropriate tissue dissociation kit, and MACS® MicroBeads.
CD11c+ DC isolation and subset analysis
Pan DCs can be isolated from dissociated tissues such as spleen, lung, or lamina propria using CD11c MicroBeads UltraPure. Enriched DCs show high viability and preserved epitopes enabling the detailed analysis of distinct subsets using MACS® antibodies.
Video
Isolation of CD11c+ dendritic cells from mouse spleen
Watch how easy it is to manually separate DCs from mouse tissue using MACS Technology.
Poster
Human and mouse dendritic cell generation
Download this poster to find all DC surface markers and products you need for convenient isolation and analysis.
Efficient loading of DCs with antigens is key for antigen presentation to T cells. By using PepTivator® Peptide Pools, antigen loading has never been easier. There are several ways a dendritic cell can be loaded with an antigen. Once option is to add into culture peptides that will be phagocyted and presented on MHC molecules.
PepTivator Peptide Pools for antigen loading
We offer a broad range of PepTivator peptide pools. This extensive panel of virus- or tumor-specific antigens consists of 15-mer peptides with 11-amino-acid overlaps, covering the complete sequence of the respective antigen.
Culturing DCs together with PepTivators leads to peptide phagocytosis and presentation on MHC molecules.
Characterization of DCs can be a challenging task. Using our REAfinity™ Recombinant Antibodies, flow cytometry analysis of human and mouse primary DC populations is easy and reproducible. Download the DC quick guide and browse through the broadest portfolio on DC generation, isolation, and analysis in the field.
MBTP 40: Immunophenotyping of CD1c+ DCs from human blood using flow cytometry
- Keywords
- Flow cytometry and cell analysis
- Immunology
- lymphocytes
- human
- dendritic cells
- blood
- Antibody panel
- Application protocol
MBTP 17: Immunophenotyping of dendritic cells from mouse spleen using flow cytometry
- Keywords
- Flow cytometry and cell analysis
- Immunology
- mouse
- spleen
- lymphocytes
- dendritic cells
- Antibody panel
- Application protocol
Multicolor flow cytometric analysis of dendritic cells (DCs) from mouse spleen
- Keywords
- Sample preparation
- Flow cytometry and cell analysis
- Immunology
- mouse
- spleen
- dendritic cells
- Application protocol
Generation of Mo-DCs
- Keywords
- Cell separation
- Cell culture
- Flow cytometry and cell analysis
- Immunology
- human
- PBMCs
- monocytes
- dendritic cells
- Application protocol
Isolation and analysis of tissue-derived CD11c+ mouse dendritic cells
- Keywords
- Sample preparation
- Cell separation
- Flow cytometry and cell analysis
- Immunology
- mouse
- spleen
- lung
- intestine
- dendritic cells
- Application protocol
