Isolation and analysis of dendritic cells and their subsets

Application protocol

Discover our different workflows for DC subsets and find the one that fits your experimental needs.

Application data by workflow step

DCs are the sentinels of the immune system and are especially located at the interface between the body and external environment. Isolation of DCs from different tissues can be performed by using the combination of gentleMACS™ Octo Dissociator with Heaters, the appropriate tissue dissociation kit, and MACS® MicroBeads.

Efficient loading of DCs with antigens is key for antigen presentation to T cells. By using PepTivator® Peptide Pools, antigen loading has never been easier.

There are several ways a dendritic cell can be loaded with an antigen. Once option is to add into culture peptides that will be phagocyted and presented on MHC molecules. 

Characterization of DCs can be a challenging task. Using our REAfinity™ Recombinant Antibodies, flow cytometry analysis of human and mouse primary DC populations is easy and reproducible. Download the DC quick guide and browse through the broadest portfolio on DC generation, isolation, and analysis in the field.