Lineage-specific chimerism analysis | MACS Cell Separation

MACSprep™ Chimerism MicroBeads allow the fast and easy isolation of target cells for
chimerism analysis. Get B cells, T cells, NK cells, myeloid cells, monocytes, granulocytes, and stem cells directly from whole blood without density gradient centrifugation or erythrocytes lysis.

The enriched cells are well-suited for lineage-specific chimerism analysis, PCR, STR analysis, flow cytometric analysis, and functional studies. In combination with the autoMACS® Pro Separator, cell isolations can be automated allowing standardized procedures with minimal hands-on time for developing routine analyses.

Basic principle

Basic principle of MACSprep™ Chimerism MicroBeads

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MACSprep™ Chimerism MicroBeads have been developed for the positive selection of cells directly from anticoagulated whole blood.

Isolate target cells directly from blood samples in three easy steps:

1. Magnetic labeling: Cells are magnetically labeled directly in the sample with MACSprep Chimerism MicroBeads.

2. Magnetic separation: Labeled cells are retained within the column. The flow-through fraction, containing erythrocytes and non-target cells, can be collected as the negative fraction.

3. Elution of labeled cells: The column is removed from the separator. The retained cells are eluted as the enriched, positively selected target cells.

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Video
Direct cell isolation from blood or bone marrow with the autoMACS® Pro Separator

With the autoMACS® Pro Separator, cells for chimerism analysis can be automatically isolated from whole blood. Take this short instrument tour and see how your target cells are being separated without the need for density gradient centrifugation or red blood cell lysis.

MACSprep™ Chimerism MicroBeads

Depending on your target cells, you can choose between a broad range of MACSprepTM Chimerism MicroBeads.

MACSprep Chimerism MicroBeads

Cell lineage	Recommended products	Order number
T cells	MACSprep Chimerism CD3 MicroBeads	130-111-551
B cells	MACSprep Chimerism CD19 MicroBeads	130-111-547
NK cells	MACSprep Chimerism CD56 MicroBeads	130-111-553
Myeloid cells	MACSprep Chimerism CD33 MicroBeads	130-111-546
Monocytes	MACSprep Chimerism CD14 MicroBeads	130-111-549
Granulocytes	MACSprep Chimerism CD15 MicroBeads MACSprep Chimerism CD66b MicroBeads	130-111-548 130-111-552
HSCs	MACSprep Chimerism CD34 MicroBead Kit	130-120-673