NK cell isolation, expansion and analysis

With this workflow, you can rely on fast and easy isolation of pure NK cells or NK cell subsets and superior NK cell expansion. Furthermore, it uses optimized flow cytometry reagents for phenotyping and functional analyses.

Whether you require highly purified populations isolated by positive selection or untouched cells from whole blood, MACS Technology has a solution for you. The NK Cell Isolation Kit, human enables you to isolate highly pure, untouched NK cells from human PBMCs.

Obtain any NK cell subset with REAlease® CD56 MicroBead Kit

If you are looking for a solution to isolate multiple NK cell subsets, REAlease® CD56 MicroBead Kit is the perfect solution for you.

• Isolation of specific CD56⁺ cell subsets. The CD56⁺ cells can be used to isolate specific NK or T cell subsets such as, CD8⁺ CD56⁺, CD3⁺ CD56⁺, CD3^- CD56⁺, and CD56⁺ CD69⁺ cell subsets.
• Isolation of label-free CD56⁺ cell subset.

As the final step in many workflows, flow analysis plays an important part in many downstream assays during NK cell analysis. The following flow reagents can ensure that the data you generate on your flow cytometer is reliable and consistent:

• REAfinity Recombinant Antibodies – recombinantly generated antibodies that ensure lot-to-lot consistency and eliminate background signal
• MACSplex Cytokine Kits – analysis of multiple soluble cytokines in one sample using any standard flow cytometer
• Cytokine Secretion Assay – Detection Kits – flow analysis of cytokine secretion at a detection level of one NK cell in a million

Build your own NK cell flow panel with our Flow Panel Builder.