NK cell isolation, expansion and analysis

Application protocols

Discover our NK cell workflows and find the one that fits your experimental needs:

Magnetic NK cell isolation

Whether you require highly purified populations isolated by positive selection or untouched cells
from whole blood, MACS Technology has a solution for you.

The NK Cell Isolation Kit, human enables you to isolate highly pure, untouched NK cells from human PBMCs.
 

Obtain any NK cell subset with REAlease® CD56 MicroBead Kit

If you are looking for a solution to isolate multiple NK cell subsets, REAlease® CD56 MicroBead Kit is the perfect solution for you.

• Isolation of specific CD56⁺ cell subsets. The CD56⁺ cells can be used to isolate specific NK or T cell subsets such as, CD8⁺ CD56⁺, CD3⁺ CD56⁺, CD3^- CD56⁺, and CD56⁺ CD69⁺ cell subsets.
• Isolation of label-free CD56⁺ cell subset.

Having trouble expanding NK cells?

Ex vivo cultivation is an attractive option to increase NK cells in numbers; either to study their biology or to improve their anti-tumor potential. But we know how difficult it is, that’s why we have developed the new NK MACS Medium. A designed formulation that offers the perfect solution to expand and activate human NK cells from various sources, including peripheral blood mononuclear cells (PBMCs) or isolated NK cells. Its defined formulation enables expansion of NK cells that are fully functional, ready for any downstream application.
 

Superior expansion of NK cells 

Reliable activation and expansion of NK cells is a clear requirement for effective downstream experiments. NK MACS® Medium is optimized for NK cell expansion and is the perfect solution to culture NK cells in vitro. 
 

Translating your research into the clinic?

Easily translate your research NK cell culture protocol into a clinical-grade protocol using the new NK MACS GMP Medium (Phenol Red), together with our MACS GMP Cytokines.

Multiparameter flow analysis of NK cells

As the final step in many workflows, flow analysis plays an important part in many downstream assays during NK cell analysis. The following flow reagents can ensure that the data you generate on your flow cytometer is reliable and consistent:

• REAfinity Recombinant Antibodies – recombinantly generated antibodies that ensure lot-to-lot consistency and eliminate background signal
• MACSplex Cytokine Kits – analysis of multiple soluble cytokines in one sample using any standard flow cytometer
• Cytokine Secretion Assay – Detection Kits – flow analysis of cytokine secretion at a detection level of one NK cell in a million

Build your own NK cell flow panel with our Flow Panel Builder.