Solutions for stimulation, enrichment, and analysis of rare virus-specific T cells 

Antigen-specific T cells play a pivotal role in fighting viral infections. Assessment of specificity, phenotype, and functional cytokine secretion of virus-activated T cells provides essential information on infection progression and the reactivity of the immune system.

However, assessment of ag-specific T cells is challenging as they constitute an extremely rare subset. Thus, a precise and sensitive analytic means is indispensable. 

Miltenyi Biotec offers several innovative products, protocols, and webinars to support you at every step of your workflow and accelerate your research
 

In vitro stimulation of  T cells with peptide pools

Enrichment of antigen-specific T cells

Flow analysis of antigen-specific T cells

  • Rapid Cytokine Inspector Kits are unique tools that combine surface marker and intracellular cytokine staining in an easy and fast way, while allowing precise analyses.
  • MACSplex Assays can be used if you are interested in analysis of multiple soluble cytokines in one sample.
  • MACS® Cytokine Secretion Assays are kits that enable you to detect and enrich viable cytokine-screening T cells at the level of one in a million, which is especially useful when viable antigen-specific T cells are needed for further down-stream applications.
  • The 8-Color Immunophenotyping Kit, human is a pre-titrated antibody cocktail which offers the most reliable immunophenotyping in a variety of samples. This enables you to identify human immune cell subsets such as monocytes, neutrophils, eosinophils, T cells, B cells, and NK cells in one sample – quickly, easily, and reproducibly.

REAfinity antibodies for flow analysis of  T cells

By performing multicolor flow analysis COVID-19 Zheng et al. recently reported that elevated exhaustion levels and reduced functional diversity of T cells in peripheral blood of COVID-19 patients may predict severe progression (PMID: 32203186).

Benefit for our REAfinity antibodies listed below to improve and simplify your flow cytometric analysis of ag-specific T cells.  
 

Related products:
Related PDFs:

Webinar: Working with antigen-specific T cells

Presenter: Dr. Marcello Stein

 

Learn in this webinar about our complete antigen-specific T cell portfolio including virus-specific application examples and publications in the field.

 

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For research requiring isolation of Pan T cells and T cell subsets
 

Untouched isolation of T cells and T cell subsets 

Direct isolation of T cells from blood and blood products

StraightFrom MicroBeads allow direct isolation of T cells from whole blood and blood products (Buffy Coat, LeukoPak® and LRSC) for e.g.  antigen-specific presentation in a Mixed Lymphocyte Reaction (MLR).

 

Related PDFs:
Related products:

Ficoll free solution to isolate PBMCs from whole blood

MACSPrep PBMC Isolation Kit, human

Check out more supporting materials on antigen-specific T cells

Rapid antigen-reactive T cell enrichment (Rapid ARTE) - Workflow protocol
Protocol

Rapid antigen-reactive T cell enrichment (Rapid ARTE)
Application

Antigen-specific T cells
Isolation of CD8+ antigen-specific T cells with the MACSQuant Tyto
Application note

Isolation of CD8+ antigen-specific T cells using the  MACSQuant® Tyto® Cell Sorter

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