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Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
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Miltenyi Biotec-tested panel 24 (MBTP 24)
This application protocol describes the flow cytometric analysis of ILC2 subsets after dissociation of mesentery and small intestine from healthy C57BL/6 mice. Mouse mesentery and small intestine are easily dissociated using the gentleMACS™ Dissociators to quickly obtain viable single cell suspensions ready for downstream applications, such as flow cytometric analysis.
Table 1: Antibody panel
Specificity | Clone | Purpose | Fluorochrome | Detection filter (nm) (laser) |
CD3 | 17A2* | Lineage | Biotin | - |
CD4 | REA604 | Lineage | Biotin | - |
CD8a | 53-6.7* | Lineage | Biotin | - |
CD11c | REA754 | Lineage | Biotin | - |
FcεRIα | MAR-1* | Lineage | Biotin | - |
NK1.1 | PK136* | Lineage | Biotin | - |
CD19 | REA749 | Lineage | Biotin | - |
Ter-119 | REA847 | Lineage | Biotin | - |
CD5 | REA421 | Lineage | Biotin | - |
F4/80 | REA126 | Lineage | Biotin | - |
Gr-1 | RB6-8C5* | Lineage | Biotin | - |
Biotin | REA746 | - | APC | 655-730 (red) |
Sca-1 | REA422 | ILC2 | Vio® Bright FITC | 525/50 (blue) |
KLRG1 | 2F1* | ILC2 | PE | 585/40 (blue) |
CD90.2 | 30-H12* | ILC2 | PerCP-Vio 770 | 655-730 (blue) |
CD45.2 | 104-2* | Lymphocytes | APC-Vio 770 | 750LP (red) |
Propidium Iodide Solution | - | Viability | - | 655-730 (blue) |
*alternative REAfinity™ Antibodies available (refer to table 2)
Table 2: Alternative REAfinity Antibodies
Specificity | Clone | Purpose | Fluorochrome | Detection filter (nm) (laser) |
CD3 | REA641 | Lineage | Biotin | - |
CD8a | REA601 | Lineage | Biotin | - |
FcεRIα | REA1079 | Lineage | Biotin | - |
NK1.1 | REA1162 | Lineage | Biotin | - |
Gr-1 | REA810 | Lineage | Biotin | - |
KLRG1 | REA1016 | ILC2 | PE | 585/40 (blue) |
CD90.2 | REA1167 | ILC2 | PerCP-Vio 770 | 655-730 (blue) |
CD45.2 | REA1223 | Lymphocytes | APC-Vio 770 | 750LP (red) |
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