Clone:
17A2
Type of antibody:
Primary antibodies, Functional-grade antibodies
Isotype:
rat IgG2bκ
Applications:
FC, FA
Alternative names:
CD3e, CD3epsilon, T3E, T3

Extended validation for CD3ε Antibody, anti-mouse

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with 17A2
145-2C11+
500A2+
REA641++
REA606+
Cells were incubated with an excess of purified unconjugated CD3ε (17A2) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD3ε. Splenocytes from BALB/c mice were stained with CD3ε antibodies and with a suitable counterstaining. As a control, CD3ε antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD3ε. Splenocytes from BALB/c mice were stained with CD3ε antibodies and with a suitable counterstaining. As a control, CD3ε antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD3ε. Splenocytes from BALB/c mice were stained with CD3ε antibodies and with a suitable counterstaining. As a control, CD3ε antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD3ε. Splenocytes from BALB/c mice were stained with CD3ε antibodies and with a suitable counterstaining. As a control, CD3ε antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD3ε. Splenocytes from BALB/c mice were stained with CD3ε antibodies and with a suitable counterstaining. As a control, CD3ε antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD3ε (17A2). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD3ε (17A2). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD3ε (17A2). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD3ε Antibody, anti-mouse

Overview

The monoclonal antibody 17A2 reacts with mouse CD3ε, a part of the CD3 complex and a subunit of the TCR complex, which is expressed on all mature T lymphocytes, NKT cells, and during the development of thymocytes. Binding of 17A2 antibody to CD3 induces cell activation and proliferation.

Alternative names

CD3e, CD3epsilon, T3E, T3

Detailed product information

Technical specifications

Clone17A2
Clonalitymonoclonal
Isotyperat IgG2bκ
Isotype controlIsotype Control Antibody, rat IgG2b
Hostrat
Type of antibodyPrimary antibodies, Functional-grade antibodies
Speciesmouse
AntigenCD3ε
Alternative names of antigenCD3e, CD3epsilon, T3E, T3
Molecular mass of antigen [kDa]19
Distribution of antigenNK cells, T cells, thymocytes
Entrez Gene ID12501
RRIDAB_2857500, AB_2811487, AB_2819753, AB_2657901, AB_2657918, AB_2751589, AB_2801708, AB_2751568, AB_2727971, AB_2751635

Resources for CD3ε Antibody, anti-mouse

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD3ε Antibody, anti-mouse

Publications

  1. Miescher, G. C. et al. (1989) Production and characterization of a rat monoclonal antibody against the murine CD3 molecular complex. Immunol. Lett. 23(2): 113-118
  2. Exley, M. et al. (1991) Structure, assembly and intracellular transport of the T cell receptor for antigen. Semin. Immunol. 3(5): 283-297

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