Optics |
| Laser excitation | Spatially separated:
405 nm, 65 mW
488 nm, 50 mW
640 nm, 72 mW |
| Emission detectors | FSC: 488/10 nm
SSC: 405/10 nm
B1: 525/50 nm
B2: 579/34 nm
B3: 615/20 nm
B4: 667/30 nm
B5: 725/40 nm
B6: 785/62 nm
V1: 450/50 nm
V2: 525/50 nm
V3: 579/34 nm
V4: 615/20 nm
V5: 667/30 nm
R1: 667/30 nm
R2: 725/40 nm
R3: 785/62 nm |
| Fluorescence sensitivity and resolution | MESFs (CV <5%):
FITC < 110
PE < 75
APC < 100 |
| Flow cell dimensions | 200×250 μm |
| Fluorescence detectors | Optimized with spectrally matched PMTs for all channels |
Fluidics |
| Minimal uptake volume1 | 1 μL (25 μL recommended for volumetric counting applications) |
| Sample flow rate | 25, 50, or 100 µL/min or automate flow rate to maintain 500, 1000, or 2000 events/second |
| Measurement speed2,3 | <25 minutes per 96-well plate (5 μL measurement volume per well) |
| Sample uptake | 1–450 µL |
| Maximal event rate | Up to 15,000 events/second |
Technical specifications | |
| Footprint | 385.5×284.5 mm (15.18×11.2 in) |
Size (width×depth)
Height (adjustable touchscreen) | 669×400 mm (26.34×15.75 in)
394–553.5 mm (15.51–21.79 in) |
| Size with miniSampler Plus (width×depth) | 669×500 mm (26.34×19.69 in) |
| Weight | 50 kg (110 lbs) |
| Monitor | 15.6" LCD touchscreen |
| Power requirements | 100–240 V~, 50/60 Hz |
| Power consumption (standard use) | 450 W |
| RAM | 8 GB DDR4 (SO-DIMM) |
| Mass storage | 500 GB SSD |
| Ports | 4× USB 2.0 ports, 6× USB 3.0 ports (2 at display),
2× DisplayPort, 2× LAN, DVI, RE-232, Audio |
| Emission sound pressure level at workstation | <61 dB(A) |
Performance |
| Sample carryover2,4 | 0.01% |
| Fluorescence performance | 5-decade logarithmic scale (10-2 to 103), displayed in lin, log, or hlog scales |
Data management | |
| Operating system | Embedded operating system |
| Measurement parameters | Area, width, height for all parameters, with time and volume |
| Signal processing | >18 bit dynamic range in area with 32 bit floating point signal processing |
| Compensation | Automated, or manual with 14×14 matrix, during or after acquisition |
| Data files | .mqd (proprietary file format), .fcs (2.0, 3.0, 3.1 compatible) |
1 At every uptake, an additional excess volume is aspirated by the instrument. The excess volumes are calibration- and process-dependent and do not exceed 10 μL for Fast, Standard, and Extended modes, and 20 μL for Screen mode.
2 Referred value indicates the average of multiple experiments and can differ for individual sample materials.
3 The measurement speed is determined by measuring the time between the movement of the robotic arm into the first measured well, and its movement out of the last measured well. The measurements were carried out at high flow rate in fast mode.
4 For carryover, full 96-well plates were loaded with 200 μL/well of PBMC suspension at a nominal concentration of 10,000 cells/μL in every other well ("SRC-wells”). Alternating wells are loaded with an equal volume of MACSQuant® Running Buffer ("CO-wells”). The uptake volume was set to 100 μL and measured at medium flow rate in standard mode. The carryover is defined by sum (CO-singlet count)/sum(SRC-singlet count) ×100%. |
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