Clone:
2F1
Type of antibody:
Primary antibodies
Isotype:
hamster IgG1κ
Alternative names:
2F1-Ag, MAFA, MAFA-L

Extended validation for KLRG1 Antibody, anti-mouse

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with 2F1
2F1/KLRG1++
REA1016++
Cells were incubated with an excess of purified unconjugated KLRG1 (2F1) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using KLRG1 (2F1). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using KLRG1 (2F1). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using KLRG1 (2F1). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for KLRG1 Antibody, anti-mouse

Overview

Killer cell lectin-like receptor G1 (KLRG1) is a homodimeric type II transmembrane glycoprotein of 30–38 kDa subunits. The protein is the mouse homolog of the rat mast cell function–associated antigen (MAFA) and is expressed on a subset of NK cells and T cells. Several members of the cadherin family can interact with KLRG1 leading to inhibition of NK cell and T cell effector functions.

Alternative names

2F1-Ag, MAFA, MAFA-L

Detailed product information

Technical specifications

Clone2F1
Clonalitymonoclonal
Isotypehamster IgG1κ
Hosthamster
Type of antibodyPrimary antibodies
Speciesmouse
AntigenKLRG1
Alternative names of antigen2F1-Ag, MAFA, MAFA-L
Molecular mass of antigen [kDa]21
Entrez Gene ID50928
RRIDAB_2652583, AB_2652584, AB_2652587