MACS® Columns at a glance

 MACS® Columns for magnetic cell isolation

  • Magnetic cell separation of highly pure and viable cells
  • Excellent recovery of target cells
  • Different column types for every cell isolation need

MACS® Columns at a glance

MACS® Columns contain a matrix composed of superparamagnetic spheres, which are covered with a cell-friendly coating. When the column is placed in a MACS Separator, the spheres amplify the magnetic field by 10.000-fold. This induces a strong magnetic field within the column that retains cells being labeled with MACS MicroBeads. Minimal labeling with only a small amount of MicroBeads is therefore sufficient to effectively separate labeled and unlabeled cells.

Inside the magnetic column, the space between the spheres is about 20 times the size of lymphocytes, so that cells can move freely and are only retained by magnetic forces. This ensures a gentle flow of cells, without pressure, sticking or compression. In consequence, cell integrity and characteristics are preserved, and the cells are fully functional for downstream applications.

Schematic view of a MACS Column. Cells move freely between the spheres inside the column and are only retained by magnetic forces.

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Choose the column you need

We offer the optimal column for your experimental set-up, desired isolation strategy, and specific application. Explore the different column options below to learn more and choose the one that fits your cell separation procedure.

For detailed information on the column type recommended for a specific reagent, please refer to the data sheet provided with the particular MACS Cell Separation Reagent.

LS Column and plunger
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Capacity

Labeled cells: up to 1×108
Total cells: up to 2×109 with manual use, up to 1×109 with MultiMACS Cell24 Separator Plus

Isolation strategy

- Positive selection
- Depletion of strongly magnetically labeled cells

Separator compatibility

MidiMACS Separator, QuadroMACS Separator, VarioMACS Separator, SuperMACS II Separator, MultiMACS Cell24 Separator Plus

MS Column and plunger
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Capacity

Labeled cells: up to 1×107
Total cells: up to 2×108

Isolation strategy

- Positive selection
- Depletion of strongly magnetically labeled cells

Separator compatibility

MiniMACS Separator, OctoMACS Separator, VarioMACS Separator, SuperMACS II Separator

LD Column and plunger
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Capacity

Labeled cells: up to 1×108
Total cells: up to 5×108

Isolation strategy

Stringent depletion of even weakly labeled cells

Separator compatibility

MidiMACS Separator, QuadroMACS Separator, VarioMACS Separator, SuperMACS II Separator, MultiMACS Cell24 Separator Plus

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Capacity

Volume whole blood: up to 15 mL with manual use, up to 10 mL with the MultiMACS Cell24 Separator Plus

Isolation strategy

Positive selection directly from human whole blood or buffy coat

Separator compatibility

MidiMACS Separator, QuadroMACS Separator, VarioMACS Separator, SuperMACS II Separator, MultiMACS Cell24 Separator Plus

* Whole Blood Columns can only be purchased with the Whole Blood Column Kit.

 

XS Column
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Capacity

Labeled cells: up to 1×109
Total cells: up to 5×108 with manual use,  up to 2×1010 with the MultiMACS Cell24 Separator Plus

Isolation strategy

Positive selection of very large cell numbers in one single step

Separator compatibility

SuperMACS II Separator

 

Large cell column
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Capacity

Labeled cells: up to 1×107
Total cells: up to 2×108

Isolation strategy

Positive selection of large human or animal cells

Separator compatibility

MiniMACS Separator, OctoMACS Separator, VarioMACS Separator, SuperMACS II Separator

 

D column with flow resistor
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Capacity

Labeled cells: up to 1×109
Total cells: up to 1×1011

Isolation strategy

Depletion of large cell numbers

Separator compatibility

SuperMACS II Separator
 

 

autoMACS® Column
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Capacity

Labeled cells: up to 2×108
Total cells: up to 4×109
Volume whole blood: up to 15 mL

Isolation strategy

- Positive selection
- Positive selection directly from human whole blood
- Depletion

Separator compatibility

autoMACS® Pro Separator
 

 

Multi-24 Column Block and 24-well Deep Well Plate
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Capacity

Labeled cells: up to 1×108 (per single column)
Total cells: up to 1×109 (per single column)

Isolation strategy

Parallel positive or untouched selection of large sample numbers or volumes

Separator compatibility

autoMACS® Pro Separator
 

 

Resources

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Brochure
MACS Cell Separation – Select the best

Download our MACS Cell Separation brochure and learn more about MACS Technology cell isolation solutions.

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