Retroviral transduction of hematopoietic stem cells (HSCs)

  • Isolation of hematopoietic stem cells
  • Retroviral transduction and in vitro expansion
  • Phenotypic flow cytometric analysis

Application data by workflow step

Isolation of hematopoietic stem cells (HSCs)

MACS® MicroBead Technology provides a broad range of marker-specific, magnetic cell enrichment solutions applicable for stem cell isolation. CD34 is a well-established marker of human hematopoietic stem and progenitor cells, which can be targeted for magnetic enrichment of this cell type. The CD34 MicroBead Kit UltraPure contains MicroBeads directly conjugated to CD34 antibodies for magnetic labeling of CD34-expressing cells from peripheral blood, cord blood, bone marrow, or apheresis harvest.
Purity of CD34+ cells isolated from a PBMC sample using the CD34 MicroBead Kit UltraPure
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CD34 MicroBead Kit UltraPure, human

  • Easy one-step isolation of CD34+ cells
  • Ideal choice for low frequency samples
  • Greatly reduces excess dead cells and debris carry-over


Retroviral transduction of HSCs

Viral transduction of hematopoietic cells is a fast, efficient method commonly used to study gene function, modulate gene expression,  or deliver a potential vector for gene correction in gene therapies. Vectofusin-1 is a fully synthetic, non-toxic cationic amphipathic peptide with viral transduction enhancing capacity, enabling high transduction levels of HSCs with low amounts of retroviral vector. Download the scientific poster below for more detailed information:

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Scientific poster
Effective retroviral transduction of primary T cells and hematopoietic stem cells using the soluble transduction enhancer Vectofusin-1

Constanze Radek1, Ornellie Bernadin2, Pia Sträßer1, Katharina Drechsel1, Andrew D.M. Kaiser1, Els Verhoeyen2,3, and Ian C. D. Johnston1

1Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany; 2CIRI – International Center for Infectiology Research, Université de Lyon, Lyon, France; 3Inserm, U1065, Centre Méditerranéen de Médecine Moléculaire (C3M), Nice, France

 In vitro expansion of HSCs

StemMACS™ HSC Expansion Media XF enables robust expansion of hematopoietic stem cells from cord blood, bone marrow, or peripheral blood. Combined with the StemMACS HSC Expansion Cocktail, a set of cytokines, this xeno-free culture medium can be used to preserve high levels of primitive CD34+CD133+ hematopoietic cells. 

StemMACS HSC Expansion Media XF maintains high levels of CD34+ cells during expansion.
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StemMACS HSC Expansion Media XF

  • Xeno-free
  • Consistently high expansion rates
  • High CD34+ cell content after expansion
MACS Handbook:

Cell culture

Related PDFs:

Flow cytometric analysis of expanded HSCs

Flow cytometric analyses provide you with quantitative information about the expression of intracellular as well as surface markers and let you assess the efficiency and quality of your viral transduction in just a few simple steps. 

Multiparameter flow analysis of HSC cells
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Multiparameter flow analysis of HSC cells

Cells expanded in StemMACS HSC Expansion Media XF maintain the characteristic surface phenotype of early hematopoietic progenitors and typically show a higher proportion of phenotypically primitive cells as compared to a competitor product.


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