Untouched isolation of PBMCs without density gradient centrifugation

  • Get untouched PBMCs directly from whole blood in 25 min 
  • Easy and fast protocol without density gradient centrifugation 
  • Pure and viable PBMCs for various downstream applications 

The MACSprep™ PBMC Isolation Kit has been developed for the fast isolation of human peripheral blood mononuclear cells (PBMCs) from 2–10 mL of freshly drawn anticoagulated whole blood without density gradient centrifugation. Isolated PBMCs are suitable for various downstream applications, such as co-cultures like functional immune cell assays, cytokine expression studies, or immune monitoring.

Basic principle

Basic principle of PBMC isolation using the MACSprep™ PBMC Isolation Kit
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With MACSprep™ PBMC Isolation Kits, untouched isolation of human PBMCs is performed in three easy steps, including labeling of non-PBMCs and a two-step separation procedure.

Data

Untouched isolation of PBMCs from human whole blood

Untouched PBMCs from high viability and purity can be isolated from human whole blood using the MACSprep™ PBMC Isolation Kit, a MACSmix™ Tube Rotator, and a LS Column

Before separation

Lysed whole blood gated on CD45+ cells

After separation

Gated on CD45+ cells
Gated on viable CD45+ cells
Gated on viable CD45+ cells
Gated on viable CD45+ CD3+ cells

Untouched PBMCs were isolated from 10 mL of human EDTA-anticoagulated whole blood using the MACSprep™ PBMC Isolation Kit, a MACSmix™ Tube Rotator, and an LS Column. The isolated cells were fluorescently stained with CD45-VioBlue® , CD3-FITC, CD4-VioGreen™, CD8-APC-Vio®  770, CD14-APC, CD16-PE, CD56-PE, CD20-PE-Vio770, and 7-AAD and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and 7-AAD.

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