European Light Microscopy Initiative Meeting 2022

European Light Microscopy Initiative Meeting 2022

June 7–10, 2022 | Turku, Finland

Understand nature’s complexity by cutting-edge imaging and microscopy technologies

Join us at ELMI 2022 to meet and exchange with our experts in all areas of antibody labelling and sample preparation, the latest microscopy technologies, and data analysis. Join us at booth #11 to collaborate with this vivid community, we can’t wait to see you in Turku, Finland! 

Join our booth and witness our innovative imaging and microscopy technologies for omni-dimensional analysis. From ultrahigh-content 2D analysis of multi-marker stained tissue, to the 3D structure visualization of full organs, and real-time 4D observation of living organisms. Our integrated and comprehensive workflow solutions make it possible! 

Logomo, Turku, Finland
Miltenyi Biotec booth #11
June 7–10 | Exhibit opening hours
Tuesday 8:30 a.m.–8:00 p.m.
Wednesday 8:30 a.m.–8:00 p.m.
Thursday  8:30 a.m.–8:00 p.m.
Friday: 8:30 a.m.–1:30 p.m.
 

Workshop

Dive deep into your sample – the Miltenyi 3D imaging workflow 

Visualizing the three-dimensional architecture of complex organisms while analyzing biological processes in a whole-organ fashion are becoming the new standard in research. To provide a complete, smooth, and hassle-free 3D imaging workflow, Miltenyi Biotec covers this entire process offering solutions for sample staining, clearing, and imaging. In this workshop, we will guide you through the most important steps of sample preparation (including a live demonstration) and demonstrate how easily high quality 3D images of large samples can be acquired with our cutting-edge light sheet system, the UltraMicroscope Blaze™.

June 8 , 2022
Workshop 1: 1:30 p.m.–2:30 p.m.
Workshop 3: 4:30 p.m.–5:30 p.m.
 
June 9 , 2022
Workshop 4: 1:30 p.m.–2:30 p.m. 
Workshop 6: 4:30 p.m.–5:30 p.m.
Logomo, Turku, Finland
Gallery 2

Agenda

What can we gain from 3D imaging? (10 minutes)
 Introduction to sample preparation (20 minutes)
3D imaging with the UltraMicroscope Blaze (20 minutes) 
VR based data visualization and analysis (10 minutes)

Speaker: Luigi Prisco, PhD

Global Product Manager, Miltenyi Biotec

Speaker information
Luigi is the Product Manager for Light sheet microscopy at Miltenyi Biotec B.V & Co. KG, Bergisch Gladbach, Germany. He conducted his PhD in Neuroscience at the German center for neurodegenerative diseases (DZNE) in Bonn, Germany between 2017-2021, after completing his Master in Biomedical Sciences at the University of Insubria, Varese, Italy. 

Speaker: Christian Gram, PhD

Microscopy Specialist, Miltenyi Biotec

Speaker information
With a passion for the field of microscopy, Christian thrives on providing technical and scientific support around advanced life science equipment to students, research professionals and professors. With his PhD studies from the Institute of Public Health (University of Southern Denmark) and Department of Molecular Biology and Genetics (Aarhus University), Christian comes as a Microscopy Specialist for Miltenyi Biotec with specializations in varying microscopy techniques and cell biology.

Speaker: Markus Habich

Group Leader and Global Product Manager, Miltenyi Biotec 

Speaker information
Dr. Markus Habich is Group Leader and Global Product Manager for imaging reagents at Miltenyi Biotec. He gained several years of experience in fluorescence microscopy applications and antibody-based assays in academia and industry. After focusing on mitochondrial biogenesis of mammalian cells at the Institute of Biochemistry, University of Cologne, Germany, Markus joined Miltenyi Biotec in 2019. In his current position, he advances the broad product portfolios of 3D imaging validated antibodies, tissue clearing reagentsand ultrahigh-content imaging antibodies for the MACSima™ Imaging Platform. 

 

Scientific poster

A novel workflow for non-toxic clearing and labelling allows for detailed high contrast three-dimensional visualization of organoids, tumor samples and whole organs 

Kevin Bigott, Johanna Brecht, Kira Gerhold, Florian Küster, Daniel Barleben, Dmytro A. Yushchenko, Lutz Haalck, Gernot Ebel, Christian Dose, Andreas Bosio, Melanie Jungblut, Lena Nolte, Dominik Eckardt

June 8, 2022
6:00 p.m.–8:00 p.m.
Logomo, Turku, Finland
Entrance/lobby area

Abstract

Generation of a 3D cellular topography of biological specimens is often essential to understand cellular and molecular processes. For a long time, biologists simply accepted the time consuming work of preparing sections, which was limited to 2D and often affected by artifacts like ripping and folding. In the last decade, clearing methods in combination with light-sheet fluorescence microscopy emerged as powerful tools for biological imaging in 3D. However, most clearing techniques are based on toxic organic solvents which require special safety provisions and generate extra effort for sample preparation as well as imaging. 

To overcome these shortcomings, we developed a novel clearing procedure based on non-toxic reagents allowing for efficient clearing of various adult mouse organs, like brain hemispheres, tumors, spheroids and organoids. Toward 3D imaging, we combined our clearing procedure with whole-mount immunostaining using Miltenyi's recombinant REAfinity™ antibodies coupled to newly developed Vio® dye variants optimized for application in light-sheet microscopy. Our data indicated highly efficient, bright and stable labelling of various structures in 3D tissue. After data acquisition, our in-house developed post processing pipeline that enables the elimination of intensity inhomogeneities and deconvolution. This allows the ideal preparation of the 3D data for subsequent data visualization and quantification. 

In summary, we developed a robust, fast, and non-toxic clearing procedure combined with bright antibody conjugates for optimal visualization of tissue in 3D, paving the way for a better understanding of tissue structures and disease mechanisms. 

Speaker: Kevin Bigott, M.Sc

Team Coordinator, Miltenyi Biotec

Speaker information
Kevin is Team Coordinator in the R&D department of neurobiology. He graduated from the University of Konstanz (Bachelor) and University of Cologne (Master) with a focus in neurobiology, imaging technology and image analysis. He joined Miltenyi in 2018 working on the implementation of new tools and applications for ultramicroscopy, including the development of a refined clearing procedure and set-up of antibody conjugates with improved features for 3D microscopy. Currently, his major interest includes the development of novel binders for advanced application in 3D imaging and new tools for deep 3D data analysis in an unobstructed environment.

Speaker: Lena Nolte, PhD

Group Leader, Miltenyi Biotec

Speaker information
Lena, once Team Coordinator within the R&D department, has progressed to Group Leader for R&D Reagents at Miltenyi Biotec B.V & Co. KG. Her academic career includes her bachelor degree at the Center for Biophotonics Science and Technology out of UC Davis, master degree at Biomolecular Photonics at Universität Bielefeld, Germany, and her PhD on multispectral imaging and second harmonic generation in scanning laser optical tomography at Leibniz Universität, Hannover. 

 

Technologies

Discover how ultra high content imaging, large-scale 3D light sheet microscopy, and multiphoton microscopy can open new avenues in cancer and immuno-oncology research.  

 

Applications and publications

Laying the foundation to understand tumor microenvironment complexity. See how researchers use technologies like ultra-high content imaging or 3D visualization by light sheet microscopy to dive deep into the biological sample and define new targets to be used in immunotherapies. 

 

Webinars and event recordings

Food for thought needed? Tune in to our webinars or watch the recordings of previous events such as the MACS Imaging and Microscopy Day. Leading scientists from the fields of immuno-oncology and neuroscience presented recent findings achieved through groundbreaking imaging and microscopy technologies. 

 

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