Tumor infiltrating B cells

In order to study tumor infiltrating B cells (TIBs), the tumor first has to be dissociated into a single cell suspension. For this, the gentleMACS™ Dissociator in combination with the Tumor Dissociation Kit, human ensures rapid and standardized dissociation of tissue samples into single-cell suspensions. Whatever the tumor entity, gentleMACS Technology provides excellent cell viability and functionality for further downstream cell separation, analysis, and culture.

Get more information on our solutions for analysis of tumor composition and microenvironment here.

To isolate tumor infiltration B cells (TIBs) positive selection positive selection is recommended, due to the highly diverse sample composition, tumor type, and origin, as well as patient differences. Whether you require for your experiments, label free B cell or classical B cell isolation using CD19 MicroBeads or cells isolated using a flow cytometer, we have a solution for you.

Magnetic B cell separation with MACS® Technology 

The amount and composition of tumor-infiltrating leukocytes (TILs) is highly variable, complicating the analysis of B cell populations. However, TIB numbers are often very low and may escape analysis when they are lost in background noise. This can be especially challenging when performing single-cell analysis. Moreover, a prerequisite for single-cell analysis is a debris-free single-cell suspensions with a viability above 80%. When working with large cohort sizes, immunophenotyping of TIBs by flow cytometry is time consuming and the data processing can be intense. Therefore, pre-enrichment is highly desirable to increase the sensitivity of analysis, saving time and effort during flow cytometry.

Microchip-based sorting of tumor infiltrating B cells 

The MACSQuant® Tyto® Cell Sorter is revolutionizing cell sorting. Our patented microchip-based technology opens up new possibilities with high-speed multiparameter cell sorting in the safety of a fully enclosed and sterile cartridge system, the MACSQuant Tyto Cartridge. This enables gentle sorting of TIBs, leading to high target cell purity and viability of theses rare cells.

Having trouble culturing B cells? 

When working with rare cell populations such as TIBs ex vivo cultivation is often used to increase B cells in numbers; either to study their biology, or transform them and analyze their antibody production as well as specificity. This can often be difficult, however using the B Cell Expansion Kit, human, streamlines and simplifies the process. The formulation offers the perfect solution to expand and activate human B cells from various sources which are fully functional and ready for any downstream application.