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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Plasma cells, through their ability to produce specific antibodies, are essential to control invading viruses and play a primary role in the adaptive immune defense. Isolation of plasma cells from virus-infected individuals can serve as a first step towards the identification of virus-specific antibodies.
The isolation procedure is fast with minimized hands-on time; it can be automated and does not require prior density gradient centrifugation and erythrocyte lysis.
Separation of CD138+ cells from a whole blood sample spiked with U266 cells, using the StraightFrom® Whole Blood and Bone Marrow CD138 MicroBeads. Cells were stained with CD45-FITC, CD138-PE, and CD19-APC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris, dead cells, and CD45– cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
| Cell type | Product | Order No |
|---|---|---|
| B cells straight from whole blood | StraightFrom Whole Blood CD19 MicroBeads, human | 130-090-880 |
| StraightFrom Whole Blood CD138 MicroBeads, human | 130-093-062 | |
| Plasma cells | CD38 MicroBead Kit, human | 130-092-263 |
| Plasma Cell Isolation Kit II, human | 130-093-628 | |
| CD138 MicroBeads, human | 130-051-301 | |
| Memory B cells | Memory B Cell Isolation Kit, human | 130-093-546 |
| IgM+ Memory B Cell Isolation Kit, human | 130-093-619 | |
| IgG+ Memory B Cell Isolation Kit, human | 130-094-350 | |
| Total B cells | Pan B Cell Isolation Kit, human | 130-101-638 |
| CD19 MicroBeads, human | 130-050-301 | |
| CD20 MicroBeads, human | 130-091-104 | |
| REAlease CD19 MicroBead Kit, human | 130-117-034 |
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