Clone:
HB14
Type of antibody:
Primary antibodies, Functional-grade antibodies
Isotype:
mouse IgG1κ, mouse IgG1
Applications:
FC, MICS, IF, IHC, ICC, FA, MC
Alternative names:
Bp50, CDW40, TNFRSF5, p50

Extended validation for CD40 Antibody, anti-human

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD40 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD40-PE (HB14, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD40 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD40-PE (HB14, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD40 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD40-PE (HB14, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD40 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD40-PE, clone (HB14). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD40 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD40-PE, clone (HB14). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD40 Antibody, anti-human

Overview

The HB14 monoclonal antibody reacts with human CD40, a 45–50 kDa type I transmembrane glycoprotein and member of the tumor necrosis factor receptor (TNFR) superfamily. It is expressed primarily on B cells, macrophages, dendritic cells, endothelial cells, and fibroblasts and at lower levels on plasma cells and a subset of peripheral T cells. CD40 is involved in B cell differentiation and proliferation, isotype class-switching, and protection of B cells from apoptosis. Interaction of CD40 with its ligand CD154 is important in T cell–B cell interaction and plays a role in co-stimulation and immune regulation. Clone HB14 blocks the binding of CD40 to CD154.

Alternative names

Bp50, CDW40, TNFRSF5, p50

Detailed product information

Technical specifications

CloneHB14
Clonalitymonoclonal
Isotypemouse IgG1κ, mouse IgG1
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies, Functional-grade antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
cynomolgus monkey (
Macaca fascicularis
)
AntigenCD40
Alternative names of antigenBp50, CDW40, TNFRSF5, p50
Molecular mass of antigen [kDa]28
Distribution of antigenB cells, dendritic cells, endothelial cells, epithelial cells, fibroblasts, Langerhans cells, lymphocytes, macrophages, mast cells, monocytes, platelets, leukemia cells, plasma cells, B cells, endothelial cells, epithelial cells, dendritic cells, fibroblasts, Langerhans cells, mast cells, monocytes, platelets, lymphocytes, leukemia cells, plasma cells, B cells, dendritic cells, endothelial cells, epithelial cells, fibroblasts, Langerhans cells, macrophages, mast cells, monocytes, platelets, lymphocytes, leukemia cells, plasma cells
Entrez Gene ID958
RRIDAB_2728026, AB_2811578, AB_2811547, AB_2811582, AB_2811556, AB_10839704, AB_2660897, AB_10828550, AB_2728049

Resources for CD40 Antibody, anti-human

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD40 Antibody, anti-human

Publications

  1. Kansas, G. S. et al. (1991) Transmembrane signals generated through MHC class II, CD19, CD20, CD39, and CD40 antigens induce LFA-1-dependent and independent adhesion in human B cells through a tyrosine kinasedependent pathway. J. Immunol. 147(12): 4094-4102
  2. Banchereau, J. et al. (1994) The CD40 antigen and its ligand. Annu. Rev. Immunol. 12: 881-926

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