Enrichment and detection of circulating tumor cells

Circulating tumor cells (CTCs) and disseminated tumors cells (DTCs) are difficult to detect and enrich. Therefore, sensitive methods for successful downstream experiments are of great interest. This innovative workflow allows for CTC and DTC enrichment and detection from whole blood, buffy coat, or PBMCs. It offers the possibility to carry out combined enrichment and staining of EpCAM+, ErbB-2+, or MCSP+ tumor cells. Alternatively, you can effectively carry out depletion of non-tumor cells

Xenografted human circulating tumor cells from peripheral mouse blood
Xenografted human CTCs from peripheral mouse blood before and after mouse cell depletion

Xenografted CTC enrichment from mouse blood

When working with patient-derived xenografts for CTC or DTC studies, human tumor cells within blood or bone marrow can be enriched without knowledge of the tumor specific marker(s) by depleting mouse cells. This enables more detailed characterization of the tumor cells by e.g. flow cytometric or morphogical analysis.

Xenografted human circulating tumor cells from peripheral mouse blood
Enriched human CD326+ tumor cells from whole blood (Data courtesy of Klaus Pantel Lab - UKE Hamburg)

CTC enrichment using CD326 (EpCAM) MicroBeads

Using the StraightFrom Whole Blood CD326 (EpCAM) MicroBeads, DTCs can be enriched within 20 minutes from whole blood samples and then analyzed by immunofluorescence microscopy and Cytokeratin+ staining.

Analysis of rare cells can be greatly improved by magnetic pre-enrichment. The MACSQuant® Analyzer 10 facilitates both automated enrichment and flow cytometric analysis. This allows the rapid and reproducible identification, quantification, and characterization of CTCs.

Scientific report
High-sensitivity detection and analysis of rare cells by integrated magnetic enrichment and flow cytometry using the MACSQuant Analyzer

Dorothee Köhler, Petra Bacher, Alexander Scheffold, Mario Assenmacher, and Manuela Herber
Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany

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