Optics |
| Laser excitation | Spatially separated:
405 nm, 40 mW
488 nm, 30 mW
640 nm, 20 mW |
| Emission detectors | FSC: 488/10 nm
SSC: 488/10 nm
V1: 450/50 nm
V2: 525/50 nm
B1: 525/50 nm
B2: 585/40 nm
B3: 655–730 nm
B4: 750 nm LP
R1: 655–730 nm
R2: 750 nm LP |
| Fluorescence sensitivity and resolution | MESFs (CV <5%):
FITC <200
PE <100
APC <150 |
| Flow cell dimensions | 200×250 μm |
| Fluorescence detectors | Optimized with spectrally matched PMTs for all channels |
Fluidics |
| Minimal uptake volume1 | 1 μL (20 μL recommended for volumetric counting applications) |
| Sample flow rate | 25–100 µL/min plus automated flow rate to maintain 500, 1000, or 2000 events/seconds |
| Measurement speed2,3 | <15 minutes for 96-well plate (5 µL per well) |
| Sample uptake | 1–5,000 µL |
| Maximal event rate | Up to 15,000 events/second |
Technical specifications | |
| Footprint | 385.5×284.5 mm (15.18×11.2 in) |
| Footprint with Orbital Shaker | 386×350 mm (15.19×13.78 in) |
Size (width×depth)
Height (adjustable touchscreen) | 669×400 mm (26.34×15.75 in)
394–553.5 mm (15.51–21.79 in) |
Size with Orbital Shaker (width×depth)
Height with Orbital Shaker (adjustable touchscreen) | 814×474 mm (32.05×8.66 in)
427–586.5 mm (16.81–23.09 in) |
| Weight | 50 kg (110 lbs) |
| Monitor | 15.6" LCD touchscreen |
| Power requirements | 100–240 V~, 50/60 Hz |
| Power consumption (standard use) | 450 W |
| RAM | 8 GB DDR4 (SO-DIMM) |
| Mass storage | 500 GB SSD |
| Ports | 4× USB 2.0 ports, 6× USB 3.0 ports (2 at display),
2× DisplayPort, 2× LAN, DVI, RE-232, Audio |
| Emission sound pressure level at workstation | <61 dB(A) |
Performance |
| Sample carry-over2,4 | 0.1% |
| Fluorescence performance | 5-decade logarithmic scale (10-2 to 103), displayed in lin, log, or hlog scales |
Data management | |
| Operating system | Embedded operating system |
| Measurement parameters | Area, width, height for all parameters, with time and volume |
| Signal processing | >18 bit dynamic range in area with 32 bit floating point signal processing |
| Compensation | Automated, or manual with 8×8 matrix, during or after acquisition |
| Data files | .mqd (proprietary file format), .fcs (2.0, 3.0, 3.1 compatible) |
1 At every uptake, an additional excess volume is aspirated by the instrument. The excess volumes are calibration- and process-dependent, and do not exceed 15 µL.
2 Referred value indicates the average of multiple experiments and can differ for individual sample materials.
3 The measurement speed is determined by measuring the time between the movement of the robotic arm into the first measured well, and its movement out of the last measured well. The measurements were carried out at high flow rate in fast mode.
4 For carry-over, full 96-well plates were loaded with 200 μL/well of PBMC suspension at a nominal concentration of 5,000 cells/μL in every other well ("SRC-wells”). Alternating wells are loaded with an equal volume of MACSQuant® Running Buffer ("CO-wells”). The uptake volume was set to 50 μL at medium flowrate. Plate shaking was set to 1200 rpm and a singlet gate was used to determine singlet counts in originating wells as well as in carry-over wells. The carry-over is defined by sum (CO-singletcount)/sum(SRC-singletcount) ×100%. Results for 384-well plates do not differ significantly from those measured in 96-well plates. |
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