The MACSprep™ PBMC Isolation Kit has been developed for the fast isolation of human peripheral blood mononuclear cells (PBMCs) from 1-8 mL of freshly drawn anticoagulated whole blood without density gradient centrifugation. Isolated PBMCs are suitable for various downstream applications, such as co-cultures like functional immune cell assays, cytokine expression studies, or immune monitoring.
Untouched PBMCs were isolated from 10 mL of human EDTA-anticoagulated whole blood using the MACSprep™ PBMC Isolation Kit, a MACSmix™ Tube Rotator, and an LS Column. The isolated cells were fluorescently stained with CD45-VioBlue® , CD3-FITC, CD4-VioGreen™, CD8-APC-Vio® 770, CD14-APC, CD16-PE, CD56-PE, CD20-PE-Vio770, and 7-AAD and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and 7-AAD.