Immunophenotyping of CAR T cell transduction efficiency and viability using flow cytometry

Miltenyi Biotec-tested panel 43 (MBTP 43)

This application protocol describes the analysis of the surface expression of chimeric antigen receptors (CARs) on T cells after transduction. To demonstrate the state-of-the-art panel, CAR T cells were generated within 12 days using the CliniMACS Prodigy® T Cell Transduction process. The data was analyzed using Miltenyi Biotec’s automated data acquisition and analysis Express Mode program “CAR T Cell Express Mode Package”.

Protocol

Materials

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