Clone:
REA734
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC, MC, 3D-IF
Alternative names:
Leu-2, CD8a, MAL, p32, Cd8b1, LY3, Lyt3, P37, Ly-2
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Human peripheral blood mononuclear cells (PBMCs) were stained with CD8 antibodies or with the corresponding REA Control (S) antibodies (left images) as well as with CD3 antibodies. Flow cytometry was performed using the MACSQuant® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.
3D-immunofluorescence analysis of human ovarian cancer fixed with 4% PFA and subjected to whole-mount immunostaining with CD8 Antibody, anti-human, Vio R667 (red). The sample was processed and optically cleared using the MACS® Clearing Kit and imaged with an UltraMicroscope Blaze light sheet instrument. Sample detail image (A), 3D-rendering (B) and ) and 3D-rendered video. Scale bars: 100 µm (A), 50 µm (C).
| Other clones | Overlap in epitope recognition with REA734 |
|---|---|
| 37006 | ++ |
| OKT8 | ++ |
| BW135/80 | + |
| HIT8a | ++ |
| REAL100 | ++ |
| RPA-T8 | - |
| SK1 | ++ |
| Cells were incubated with an excess of purified unconjugated CD8 (REA734) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker. | |
| Clone | REA734 |
|---|---|
| Clonality | monoclonal |
| Isotype | recombinant human IgG1 |
| Isotype control | REA Control Antibody (S), human IgG1 |
| Host | human cell line |
| Type of antibody | Primary antibodies, Recombinant antibodies |
| Species | human |
| Antigen | CD8 |
| Alternative names of antigen | Leu-2, CD8a, MAL, p32, Cd8b1, LY3, Lyt3, P37, Ly-2 |
| Molecular mass of antigen [kDa] | 45 |
| Distribution of antigen | NK cells, T cells |
| Entrez Gene ID | 925, 926 |
| RRID | AB_2659233, AB_2659234, AB_2659235, AB_2659236, AB_2659237, AB_2659238, AB_2659239, AB_2659240, AB_2659241, AB_2659242, AB_2659243, AB_2659244, AB_2659245, AB_2659246, AB_2659247, AB_2659248, AB_2659249, AB_2751050, AB_2751048, AB_2659250, AB_2659251, AB_2801862, AB_2922105, AB_2659232 |
This anti-CD8 antibody was tested in order to establish a panel to describe and distinguish peripheral blood lymphocytes (from human PBMCs). Clone REA734 recognizes the human CD8 antigen, a cell-surface glycoprotein also known as Leu-2 or CD8a. We are particularly interested in the innate compartment of lymphocytes and we use CD8 to target, and also exclude, a particular subset of T lymphocytes.
| Other clones | Overlap in epitope recognition with REA734 |
|---|---|
| 37006 | ++ |
| OKT8 | ++ |
| BW135/80 | + |
| HIT8a | ++ |
| REAL100 | ++ |
| RPA-T8 | - |
| SK1 | ++ |
| Cells were incubated with an excess of purified unconjugated CD8 (REA734) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker. | |
| Clone | REA734 |
|---|---|
| Clonality | monoclonal |
| Isotype | recombinant human IgG1 |
| Isotype control | REA Control Antibody (S), human IgG1 |
| Host | human cell line |
| Type of antibody | Primary antibodies, Recombinant antibodies |
| Species | human |
| Antigen | CD8 |
| Alternative names of antigen | Leu-2, CD8a, MAL, p32, Cd8b1, LY3, Lyt3, P37, Ly-2 |
| Molecular mass of antigen [kDa] | 45 |
| Distribution of antigen | NK cells, T cells |
| Entrez Gene ID | 925, 926 |
| RRID | AB_2659233, AB_2659234, AB_2659235, AB_2659236, AB_2659237, AB_2659238, AB_2659239, AB_2659240, AB_2659241, AB_2659242, AB_2659243, AB_2659244, AB_2659245, AB_2659246, AB_2659247, AB_2659248, AB_2659249, AB_2751050, AB_2751048, AB_2659250, AB_2659251, AB_2801862, AB_2922105, AB_2659232 |
This anti-CD8 antibody was tested in order to establish a panel to describe and distinguish peripheral blood lymphocytes (from human PBMCs). Clone REA734 recognizes the human CD8 antigen, a cell-surface glycoprotein also known as Leu-2 or CD8a. We are particularly interested in the innate compartment of lymphocytes and we use CD8 to target, and also exclude, a particular subset of T lymphocytes.
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