Principle
The MultiMACS X Separator combines true walk-away convenience with high-throughput sample processing. You can now enjoy all the benefits of the MultiMACS Cell24 Separator Plus and a customized liquid handler for precise pipetting and sample transfer. Parallel processing of high sample numbers or large sample volumes ensures high standardization and reproducibility.
Play video
Background
The MultiMACS X is designed for the precise execution of any positive or untouched isolation using MACS® Cell Separation reagents, including StraightFrom® MicroBeads. Separations can be performed with the Multi-24 Column Block or with 12 or less LS, LD, or Whole Blood Columns in combination with the LHS Single-Column Adapter. The MultiMACS X contains a permanent 24-well magnet that induces a high-gradient magnetic field within columns. The field is strong enough to retain cells labeled with even small amounts of MACS MicroBeads within the columns. The MultiMACS X Chill Racks allow you to keep your samples and reagents at 2–8 °C. After transfer of the columns to the Elution Chamber of the MACS Elution Station X, magnetically labeled cells can be eluted from the columns via a small vacuum impulse. The instrument is able to take additional pipetting steps off your hands, such as aliquoting samples for further processing or labeling cells for flow cytometric analysis. Automated pipetting reduces inter-operator variability, saves time, and improves operator safety.
Documentation
With the use of a handheld barcode scanner, the MultiMACS X records barcoded information of samples, as well as tracks the lot numbers and expiry dates of MACS MicroBeads and Isolation Kits. At the end of each run, the instrument provides a short report detailing if the run was completed successfully. The software can also be integrated into a laboratory information management system (LIMS) for further documentation.
Level of automation
Fully automated
Footprint
| Dimensions (w × d × h) | 1,145 mm × 1,000 mm × 1,025 mm (from benchtop with safety panel closed) | |
|---|---|---|
| Maximum outer dimensions (w × d × h) | 1,393 mm × 1,285 mm × 1,469 mm (side door open for service accessibility, safety panel in middle position, height incl. holder for disposable waste bag) | |
| Weight | 188 kg (415 lb) dry weight 221 kg (488 lb) shipping weight | |
Order number
130-118-515
Whether following a positive selection or an untouched strategy, parallel separation is fast and provides reproducible results because all samples are treated identically. Different column options enable you to adapt to sample size, type, and separation strategy. The Multi-24 Column Block supports both positive selection and depletion. It enables high-throughput and large-volume applications by combining 24 columns in one unit. It saves time and makes handling of high sample numbers or volumes more convenient.
The LHS Single-Column Adapter is designed for use with single MACS Columns and allows you to handle up to 12 samples in one go. It is compatible with LS, LD, or Whole Blood Columns. LS Columns are optimized for positive selection and can also be used for depletion strategies. LD Columns are optimized for depletion and Whole Blood Columns for cell isolation directly from whole blood, bone marrow, and buffy coat. The Single-Column Adapter helps you save column costs when you want to process only a small number of samples in a run.
| Capacity labeled cells (max. magnetically labeled cells) | Capacity total cells (max. total cells or max. blood volume) | |
|---|---|---|
| Multi-24 Column Block | Up to 1×10⁸ (per single column) | Up to 1×10⁹ (per single column) |
| LS Column | Up to 1×108 | Up to 1×109 |
| LD Column | Up to 1×108 | Up to 5×108 |
| Whole Blood Column | NA | Up to 10 mL |
Separation strategies
| Strategy | Products |
|---|---|
| Positive separation | MACS MicroBeads and MicroBead Kits StraightFrom® MicroBeads MACSprep™ MicroBeads |
| Untouched isolation | MACS Isolation Kits |
| Indirect MicroBeads | e.g. Anti-Biotin MicroBeads |
Starting material
| Starting material | |
|---|---|
| Sample type | - PBMCs - Whole blood - Buffy coat - Leukapheresis sample - LRSC - Bone marrow - Single-cell suspensions from dissociated tissue or tumor - Cultured cells (cell lines and primary cells) |
| Species | - Human - Mouse - Rat - Non-human primate - All others possible with Indirect MicroBeads |
| Number of samples | - Up to 24 samples with the Multi-24 Column Block - Up to 12 samples with the Single-Column Adapter |
Exclusive MultiMACS X consumables
Columns
Elution and Waste Plates
Accessories
Download PDF
Brochures/posters
Scientific posters
- Workflow automation and parallelization improves the isolation and analysis of tumor-infiltrating immune cell subpopulations
- Isolation of functional cells straight from buffy coat, LRSC, and Leukopak®
- Workflow automation and parallelization improves the isolation and analysis of tumor-infiltrating immune cell subpopulations
Preinstalled base applications
Base applications are available for key cell isolation workflows. They are installed as default applications on the instrument and are ready to use.
These applications include the direct isolation of selected target cells from main blood sources (Leukopak®, Whole Blood, and Buffy Coat) with the StraightFrom® MicroBead portfolio, untouched isolation from PBMCs with MACS Isolation Kits, human, and positive selections from PBMCs with MACS MicroBeads.
The most common immune cell types (e.g. T cells, B cells, monocytes, and NK cells) are included in these base applications.
Custom application development
Customized processes for automated high-throughput cell separation on the MultiMACS X are developed and validated to match your needs perfectly and to ensure any cell separation specification can be fulfilled.
This approach enables integration of additional steps, such as sample aliquoting. With its open architecture, the MultiMACS X offers great flexibility and can be easily integrated as a fully automated cell separation module into any cell processing workflow. This successfully minimizes risk, costs, delays, and implementation time.
A dedicated Miltenyi Customized Application Development team is available to translate your individual cell separation workflow into a fully automated process by implementing custom-tailored scripts.
Selected applications
Intuitive software for tracking and documentation
- The touchscreen interface is easy to use for any lab professional and guides users through the automated cell separation process
- The intuitive graphical user interface (GUI) walks you through each step of the experiment setup and will inform you about the status of the separation process and remaining time during a run.
- Base applications for key workflows are preinstalled and an application development service is available for additional customized applications.
- Easy maintenance routines can be initiated by the user through the click of a button
Support when you need it
- Technical and field application support for application design and troubleshooting through our application development team and global technical support team.
- You can access a collection of FAQs and technical documents, including datasheets, protocols, and manuals.
- Training at Miltenyi University
- Protect your instrument with a comprehensive service contract.
MultiMACS X Separator
Walk-away cell isolation for demanding high-throughput cell processing laboratories.
Order no.: 130-118-515.
