Direct isolation of B and T cells for HLA crossmatch analysis

  • Cell isolation directly from blood or spleen in 20 min
  • Untouched isolation of B and T cells in parallel 
  • Excellent viability of target cells for reliable HLA analysis

MACSprep™ HLA Cell Isolation Kits allow the untouched isolation of human lymphocytes for optimal HLA crossmatch analysis. The fast and easy protocol allows direct cell isolation from anticoagulated whole blood or spleen cell suspensions in only 20 minutes, without any density gradient centrifugation or RBC lysis. Isolated target cells such as B, T, or B and T lymphocytes have high purity and viability, and are suited for CDC (complement dependent cytotoxicity) research.

Basic principle

Basic principle of MACSprep™ HLA Cell Isolation Kits
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MACSprep™ HLA Cell Isolation Kits allow the untouched isolation of human lymphocytes from anticoagulated whole blood or spleen.

Non-target cells are removed by immunomagnetic depletion while erythrocytes form a sediment, yielding viable untouched target cells of high purity and viability. 

Data

Reliable performance with MACSprep HLA Cell Isolation Kits. B and T cells (BT), B, or T cells were isolated from 20 different donors using the MACSprep HLA Cell Isolation Kits. 
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Isolation of B and T cells using MACSprep HLA Cell Isolation Kits

With the MACSprep HLA Cell Isolation Kits for B cells, T cells, or B and T cells, target cell purity of up to 97% and viability up to 95% can be achieved.
 

Starting kits

MACSPrep™ HLA Cell Starting Kit MACSxpress
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First time isolating cells for HLA analysis?

We offer a MACSprep HLA Starting Kit MACSxpress containing all necessary components to process up to 25 mL suspension per isolation, with two samples in parallel. 

The MACSprep HLA Starting Kit MACSiMAG contains all necessary components to process up to 16 mL suspension per sample, with three to eight samples in parallel. 

Resources

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Brochure
MACSprep™ Technology - Excellence in cell preparation for diagnostic research

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Brochure
MACSprep™ HLA Cell Isolation Kits - Quick and simple isolation of B and T cells

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Scientific poster
A simple and fast method for enrichment of lymphocyte subsets for complement-dependent cytotoxicity assays.

Adriana Salomon1 , Phillip Rutsch1 , Annette von Borstel², Matthias Könn1 , Vanessa Ditt2 , Gregor Winkels¹  
¹ Miltenyi Biotec GmbH, Bergisch Gladbach, Germany | 2 Institute for Clinical Transfusion Medicine, Transplant Center Cologne, Merheim Medical Center, Cologne General Hospital, Cologne, Germany 
 

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