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|Column||Max. number of labeled cells||Max. number of total cells||Selector|
DPBS/BSA buffer: Prepare a solution containing Dulbecco’s phosphate-buffered saline (DPBS) with Ca2+ and Mg2+ and 0.5% bovine serum albumin (BSA) by diluting MACS® BSA Stock Solution 1:20 with DPBS. Keep buffer cold (2–8 °C). Prepare fresh on the day of use and degas the buffer, as air bubbles could block the column.
Coat a 24-well culture dish with 0.01% Poly-L-lysine overnight at 37 °C. Wash three times with ddH₂O the following day.
For cell culture, prepare the following medium: MACS Neuro Medium containing 2% MACS NeuroBrew®-21, 1% penicillin/streptomycin and 0.5 mM L-glutamine.
Dissociate mouse neonatal brain using the Neural Tissue Dissociation Kit – Postnatal Neurons. Follow the protocol of the kit data sheet.
Download data sheet
Staining buffer: Prepare a solution containing autoMACS® Running Buffer with FcR Blocking Reagent, mouse (1:10).
|Primary Antibody||Secondary Antibody|
|Antibody||Incubation temperature||Incubation time||Recommended antibody concentration||Secondary antibody||Incubation temperature||Incubation time|
|Anti-ACSA-2||Room temperature||10 minutes||1–5 µg/mL||Anti-rat IgG2b||Room temperature||10 minutes|
|Anti-GLAST (ACSA-1)||Room temperature||10 minutes||1–5 µg/mL||Anti-mouse IgG2a||Room temperature||10 minutes|
|Anti-O4||Room temperature||10 minutes||5–10 µg/mL||Anti-mouse IgM||Room temperature||10 minutes|
|Anti-PSA-NCAM||Room temperature||10 minutes||5–10 µg/mL||Anti-mouse IgM||Room temperature||10 minutes|
|CD11b||Room temperature||10 minutes||5–10 µg/mL||Anti-rat IgG2bκ||Room temperature||10 minutes|
|CD68||2–8 °C||Overnight||5–10 µg/mL||Anti-rat IgG2a||Room temperature||1 hour|
|CD171 (L1CAM)||2–8 °C||Overnight||5–10 µg/mL||Anti-rat IgG2a||Room temperature||1 hour|
Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use.
autoMACS, CliniMACS, the CliniMACS logo, CliniMACS Prodigy, CryoMACS, CytoMix, CytoStim, DendriMACS, ExiTron, ExpAct, FeraSpin, FeraTrack, GadoSpin, gentleMACS, LIFE 18, LIFE 21, MACS, the MACS logo, MACSductin, MACSelect, MACSfectin, MACSflex, MACSiBead, MACSiMAG, MACSmix, MACSprep, MACSQuant, MACSQuantify, MACSxpress, MidiMACS, MiniMACS, miRXplore, MultiMACS, NeuroBrew, NiraWave, OctoMACS, PepTivator, pMACS, PolySon, PrepProtect, QuadroMACS, REAfinity, REAlease, Rheo, StemMACS, StraightFrom, SuperAmp, SuperMACS, TexMACS, TheraSorb, thermoMACS, TransAct, Tyto, the Tyto logo, VarioMACS, Vio, Viobility, VioBlue, VioBright, VioGreen, Viscover, and µMACS are registered trademarks or trademarks of Miltenyi Biotec B.V. & Co. KG and/or its affiliates in various countries worldwide. Ficoll-Paque is a trademark of GE Healthcare companies. Vectofusin-1 is a registered trademark of Genethon. All other trademarks mentioned in this document are the property of their respective owners and are used for identification purposes only. Copyright © 2019 Miltenyi Biotec B.V. & Co. KG and/or its affiliates. All rights reserved.
Copyright © 2020 Miltenyi Biotec and/or its affiliates. All rights reserved.