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Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
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Miltenyi Biotec-tested panel 20 (MBTP 20)
This application protocol describes the flow cytometric analysis of the activation status of NK cells isolated from PBMCs from whole blood of a healthy donor. Immunophenotyping of NK cells was performed on all PBMCs, directly after isolation via CD3 depletion and CD56 enrichment, and after 14 days of culture.
Channel | Specificity | Clone | Purpose | Fluorochrome | Detection filter (nm) (laser) |
V1 | CD56 | REA196 | NK cells | Vio® Bright V423 | 450/50 (violet) |
V2 | CD45 | REA747 | Leukocytes | VioGreen™ | 525/50 (violet) |
B1 | CD335 (NKp46) | REA808 | Activation marker | Vio Bright B515 | 525/50 (blue) |
B2 | CD336 (NKp44) | REA1163 | Activation marker | PE | 585/40 (blue) |
B3 | 7-AAD Staining Solution | - | Viability | - | 655–730 (blue) |
B3 | CD3 | REA613 | Cell type exclusion | PerCP-Vio 700 | 655–730 (blue) |
CD14 | REA599 | Cell type exclusion | |||
B4 | CD337 (NKp30) | REA823 | Activation marker | PE-Vio 770 | 750LP (blue) |
R1 | CD314 (NKG2D) | REA797 | Activation marker | APC | 655–730 (red) |
R2 | CD16 | REA423 | Maturation marker | APC-Vio 770 | 750LP (red) |
Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use.
autoMACS, CliniMACS, the CliniMACS logo, CliniMACS Prodigy, CryoMACS, CytoMix, CytoStim, DendriMACS, ExiTron, ExpAct, FeraSpin, FeraTrack, GadoSpin, gentleMACS, LIFE 18, LIFE 21, MACS, MACSductin, MACSelect, MACSfectin, MACSflex, MACSiBead, MACSiMAG, MACSmix, MACSprep, MACSQuant, MACSQuantify, MACSxpress, MidiMACS, the Miltenyi Biotec logo, MiniMACS, miRXplore, MultiMACS, NeuroBrew, NiraWave, OctoMACS, PepTivator, pMACS, PolySon, PrepProtect, QuadroMACS, REAfinity, REAlease, Rheo, StemMACS, StraightFrom, SuperAmp, SuperMACS, TexMACS, TheraSorb, thermoMACS, TransAct, Tyto, the Tyto logo, VarioMACS, Vio, Viobility, VioBlue, VioGreen, Viscover, and µMACS are registered trademarks or trademarks of Miltenyi Biotec and/or its affiliates in various countries worldwide. Ficoll-Paque is a trademark of GE Healthcare companies. Vectofusin-1 is a registered trademark of Genethon. All other trademarks mentioned in this document are the property of their respective owners and are used for identification purposes only. Copyright © 2020 Miltenyi Biotec and/or its affiliates. All rights reserved.
Copyright © 2021 Miltenyi Biotec and/or its affiliates. All rights reserved.