gentleMACS™ Dissociator

The gentleMACS™ Dissociator is a benchtop instrument for the semi-automated dissociation of tissues into single-cell suspensions or thorough homogenates. A single sample or two samples in parallel can be processed. Two types of unique gentleMACS Tubes used with the instrument enable the time-saving and easy dissociation or homogenization of tissues in a closed system. The instrument offers optimized gentleMACS Programs for a variety of specific applications. Special protocols have been developed for various tissues. The standardized tissue dissociation or homogenization procedures make for reliable and reproducible results.

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The gentleMACS™ Dissociator

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The gentleMACS™ Dissociator

Specifications for gentleMACS™ Dissociator

Overview

The gentleMACS™ Dissociator is a benchtop instrument for the semi-automated dissociation of tissues into single-cell suspensions or thorough homogenates. A single sample or two samples in parallel can be processed. Two types of unique gentleMACS Tubes used with the instrument enable the time-saving and easy dissociation or homogenization of tissues in a closed system. The instrument offers optimized gentleMACS Programs for a variety of specific applications. Special protocols have been developed for various tissues. The standardized tissue dissociation or homogenization procedures make for reliable and reproducible results.
For higher throughput and even more flexibility see also the gentleMACS Octo Dissociator. For fully automated tissue dissociation browse the gentleMACS Octo Dissociator with Heaters.

Detailed product information

Applications

Optimized, pre-set gentleMACS Programs have been developed for easy dissociation of tissues into single-cell suspensions, including:
  • mouse or human tumor
  • mouse or rat neonatal heart
  • neural tissue
  • mouse spleen, lung, lamina propria, muscle, epidermis, or liver
  • mouse or human skin
  • and more
Also, programs have been developed for the homogenization of tissues, for:
  • total RNA or mRNA isolation from fresh or frozen samples
  • protein extraction
  • determination of bacterial or viral load
  • mitochondria isolation

Reviews for gentleMACS™ Dissociator

Dissociate Tissues for Cell Recovery

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gentleMACS Dissociator (130-093-235)

This is basically a glorified blender that dissociates any tissue digested with collagenase, to extract infiltrating cells. It can be used for tumors, pancreases, kidneys, heart etc. Some solid tissues are difficult to dissociate by manual agitation, and this is where it comes handy. Plus, who wants to hurt their hands when you have a million tissues to process. We actually performed a yield test and found that tumors dissociated with this instrument gave 5 times higher numbers of infiltrating leukocytes than manually agitated samples.

C Tubes & GentleMACS Dissociator for Tumor Dissociation

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gentleMACS Dissociator (130-093-235)

Miltenyi Biotech's C-Tubes are designed to streamline tissue dissociation using the gentleMACS™ Dissociator, and are very helpful in doing so. By using set programs on the dissociator machine, you can select for specific cell types that you wish to isolate. I used these to dissociate tumor tissue in a cell line derivation procedure and received consistent, thoroughly positive results with my final cell lines.

C Tubes & gentleMACS Dissociator for Tumor Dissociation

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gentleMACS Dissociator (130-093-235)

Miltenyi Biotech's C-Tubes are designed to streamline tissue dissociation, and are very helpful in doing so. By using set programs on the accompanying dissociator machine, you can select for specific cell types that you wish to isolate. I used these to dissociate tumor tissue in a cell line derivation procedure and received consistent, thoroughly positive results with my final cell lines.

Perfect for Tissue Dissociation

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gentleMACS Dissociator (130-093-235)

This is a perfect instrument for tissue dissociation. Once you know it you do not want to dissociate animal tissue without it.

Single Cell Isolation From Various Tissues

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gentleMACS Dissociator (130-093-235)

Our lab focused on the clonal heterogeneity in various tissues. As part of our project, need to isolate single cells from various tissues ( liver, lung, brain) from mouse and tumors. The reason we choose MACS dissociator because it has pre-programme optimized for single cell preparations - Lung, brain and tumor.

Perfect For Dissociating Various Tissues

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gentleMACS Dissociator (130-093-235)

Our lab digests large amounts of both human and animal tissue for single cell isolation. This machine very quickly dissociates many types of tissue that cannot digest in enzyme buffer alone. We use it for lung, spleen, intestine, and lymph nodes.

gentleMACS Dissociator For Suspending Tough Tissues

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gentleMACS Dissociator (130-093-235)

My lab studies CD8 T-cell mediated immunity in the genital mucosa and so we utilize this dissociator to break up evolutionarily tough tissues to free and suspend the cells inside.

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gentleMACS Dissociators: Start smart!

Get your research off to the right start. Rely on excellent sample preparation with the gentleMACS Dissociator family. 

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