Immunophenotyping of B cells from human buffy coat using flow cytometry

MBTP 27= Miltenyi Biotec-tested panel 27

This application protocol describes the flow cytometric analysis of B cell subsets after isolation of B cells from human buffy coat. B cells were isolated from human buffy coat using the Pan B Cell Isolation Kit, human to quickly obtain viable single cell suspensions ready for downstream applications, such as flow cytometric analysis.

Protocol

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Materials

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