The CD4
+
T Cell Isolation Kit was developed for the isolation of untouched CD4
+
T cells from rhesus monkey PBMCs.

Data and images for
CD4
+
T Cell Isolation Kit
, non-human primate

Figures

Figure 1

Isolation of untouched CD4
+
T cells from rhesus monkey PBMCs using the CD4
+
T Cell Isolation Kit, a MidiMACS™ Separator, and an LS Column. Cells are fluorescently stained with CD45-FITC, CD4-PE and CD3-APC. Plots show viable CD45
+
leukocytes.
PBMCs before separation
Isolated CD4
+
T cells
View details

Figure 1

Isolation of untouched CD4
+
T cells from rhesus monkey PBMCs using the CD4
+
T Cell Isolation Kit, a MidiMACS™ Separator, and an LS Column. Cells are fluorescently stained with CD45-FITC, CD4-PE and CD3-APC. Plots show viable CD45
+
leukocytes.
View details

Figure 1

Isolation of untouched CD4
+
T cells from rhesus monkey PBMCs using the CD4
+
T Cell Isolation Kit, a MidiMACS™ Separator, and an LS Column. Cells are fluorescently stained with CD45-FITC, CD4-PE and CD3-APC. Plots show viable CD45
+
leukocytes.

Specifications for
CD4
+
T Cell Isolation Kit
, non-human primate

Overview

The CD4
+
T Cell Isolation Kit was developed for the isolation of untouched CD4
+
T cells from rhesus monkey PBMCs.

Detailed product information

Background information

Non-CD4
+
T cells are indirectly magnetically labeled by using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Highly pure untouched CD4
+
T cells are obtained by depletion of the magnetically labeled cells.
Untouched CD4
+
T cells may also be used for further isolation of specific T cell subsets. The CD4
+
CD25
+
Regulatory T Cell Isolation Kit is recommended for most convenient separation of CD4
+
CD25
+
regulatory T cells. Furthermore, the CD4
+
T Cell Isolation Kit can be used in combination with CD95-PE and Anti-PE MicroBeads to isolate naive CD4
+
T cells from non-human primate PBMCs. A special protocol for this application is available.

Downstream applications

T cells isolated with the CD4
+
T Cell Isolation Kit are used when direct labeling of T cell surface molecules could interfere with downstrem applications, e.g., in studies on T helper cell activation.

Columns

MS, LS, or autoMACS
®
Columns.

Resources for
CD4
+
T Cell Isolation Kit
, non-human primate

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CD4
+
T Cell Isolation Kit
, non-human primate

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