Anti-Phycoerythrin (PE) MicroBeads are used for the indirect magnetic labeling and separation of cells or other materials labeled with a PE-conjugated primary antibody or ligand. The use of PE-conjugated primary antibodies and Anti-PE MicroBeads leads to the highest purity and recovery, even when the marker used for sorting is weakly expressed. Anti-PE MicroBeads UltraPure have been especially designed for cell separations from debris-rich biological starting materials.

Data and images for Anti-PE MicroBeads

Figures

Figure 1

Isolation of cytomegalovirus (CMV)-specific T cells from PBMCs using PE-conjugated CMVpp65/A2 tetramers (courtesy of Prof. Moss, Birmingham, U.K.) and Anti-PE MicroBeads. Cells were positively selected by separation over two MS Columns.
PBMCs before separation
Enriched CMV-specific T cells
View details

Figure 1

Isolation of cytomegalovirus (CMV)-specific T cells from PBMCs using PE-conjugated CMVpp65/A2 tetramers (courtesy of Prof. Moss, Birmingham, U.K.) and Anti-PE MicroBeads. Cells were positively selected by separation over two MS Columns.
View details

Figure 1

Isolation of cytomegalovirus (CMV)-specific T cells from PBMCs using PE-conjugated CMVpp65/A2 tetramers (courtesy of Prof. Moss, Birmingham, U.K.) and Anti-PE MicroBeads. Cells were positively selected by separation over two MS Columns.

Figure 2

Human CLEC9a
+
cells have been isolated from a debris-rich sample of peripheral blood mononuclear cells (PBMCs) using Anti-CLEC9a-PE, Anti-PE MicroBeads UltraPure, two MS Columns, and a MiniMACS™ Separator. Cells were stained with Anti-CLEC9a-PE (# 130-097-368), CD141 (BDCA-3)-APC (# 130-090-907), and Propidium Iodide Solution (# 130-093-233). Cells were analyzed after gating on viable lymphoid cells.
Before separation
After separation
View details

Figure 2

Human CLEC9a
+
cells have been isolated from a debris-rich sample of peripheral blood mononuclear cells (PBMCs) using Anti-CLEC9a-PE, Anti-PE MicroBeads UltraPure, two MS Columns, and a MiniMACS™ Separator. Cells were stained with Anti-CLEC9a-PE (# 130-097-368), CD141 (BDCA-3)-APC (# 130-090-907), and Propidium Iodide Solution (# 130-093-233). Cells were analyzed after gating on viable lymphoid cells.
View details

Figure 2

Human CLEC9a
+
cells have been isolated from a debris-rich sample of peripheral blood mononuclear cells (PBMCs) using Anti-CLEC9a-PE, Anti-PE MicroBeads UltraPure, two MS Columns, and a MiniMACS™ Separator. Cells were stained with Anti-CLEC9a-PE (# 130-097-368), CD141 (BDCA-3)-APC (# 130-090-907), and Propidium Iodide Solution (# 130-093-233). Cells were analyzed after gating on viable lymphoid cells.

Specifications for Anti-PE MicroBeads

Overview

Anti-Phycoerythrin (PE) MicroBeads are used for the indirect magnetic labeling and separation of cells or other materials labeled with a PE-conjugated primary antibody or ligand. The use of PE-conjugated primary antibodies and Anti-PE MicroBeads leads to the highest purity and recovery, even when the marker used for sorting is weakly expressed. Anti-PE MicroBeads UltraPure have been especially designed for cell separations from debris-rich biological starting materials.

Detailed product information

Background information

Anti-PE MicroBeads UltraPure have been especially developed for highly efficient separation of cells from debris-rich samples or other biological materials according to surface markers labeled with PE-conjugated primary antibodies, peptides or ligands. After separation the PE-labeled cells can be directly detected by flow cytometry or fluorescence microscopy without any further staining. Together with its ultrapure cell separation result even from debris-rich starting material Anti-PE MicroBeads UltraPure are the latest development of Anti-PE MicroBeads.

Applications

Anti-PE MicroBeads are well-established as a component of the Cytokine Secretion Assays for the enrichment of antigen-specific T cells. They have also been used to isolate antigen-specific T cells after labeling with PE-conjugated MHC Class I tetramers
1,2
or MHC Class II tetramers
3
. Moreover, they have been used to separate a CD62L
+
subset of CD4
+
CD45RO
+
memory T cells from human adenoids, which had been pre-sorted by MACS® Technology
4
. The latest development of Anti-PE MicroBeads UltraPure enables high-yield positive cell separation using any PE-labeled primary antibody, even from low quality and rare cell samples.

Columns

For positive selection: MS, LS, XS, or autoMACS
®
Columns. For depletion: LD, D, or autoMACS Columns.

Reviews for Anti-PE MicroBeads

Anti-PE MicroBeads

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Anti-PE MicroBeads (130-048-801)

We perform both depletion and selection of multiple cell types from cell cultures as well as whole tissues including spleen, lymph nodes, thymus, lung, and bone marrow. Cell samples are then used for RT-qPCR, Flow Cytometric analysis, and re-culturing for functional studies.

References for Anti-PE MicroBeads

Publications

  1. Shankar et al. (2001) Blood 96: 3094-3101
  2. Barnes et al. (2004)
    Ultra-sensitive class I tetramer analysis reveals previously undetectable populations of antiviral CD8
    +
    T cells.
    Eur. J. Immunol. 34: 1570-1577
  3. Lucas, M. et al. (2004)
    Ex vivo
    phenotype and frequency of influenza virus-specific CD4 memory T cells.
    J. Virol. 78: 7284-7287
  4. Mattila, P. S. et al. (2000)
    Adenoids provide a microenvironment for the generation of CD4
    +
    , CD45RO
    +
    , L-selectin
    , CXCR4
    +
    , CCR5
    +
    T lymphocytes, a lymphocyte phenotype found in the middle ear effusion.
    Int. Immunol. 12: 1235-1243

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